IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Zebrafish mll Gene Is Essential for Hematopoiesis
Wan, Xiaoyang; Hu, Bo; Liu, Jing-xia; Feng, Xi; Xiao, Wuhan; Xiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; w-xiao@ihb.ac.cn
2011
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
Volume286Issue:38Pages:33345-33357
AbstractStudies implicate an important role for the mixed lineage leukemia (Mll) gene in hematopoiesis, mainly through maintaining Hox gene expression. However, the mechanisms underlying Mll-mediated hematopoiesis during embryogenesis remain largely unclear. Here, we investigate the role of mll during zebrafish embryogenesis, particularly hematopoiesis. Mll depletion caused severe defects in hematopoiesis as indicated by a lack of blood flow and mature blood cells as well as a significant reduction in expression of hematopoietic progenitor and mature blood cell markers. Furthermore, mll depletion prevented the differentiation of hematopoietic progenitors. In addition, we identified the N-terminal mini-peptide of Mll that acted as a dominant negative form to disrupt normal function of mll during embryogenesis. As expected, mll knockdown altered the expression of a subset of Hox genes. However, overexpression of these down-regulated Hox genes only partially rescued the blood deficiency, suggesting that mll may target additional genes to regulate hematopoiesis. In the mll morphants, microarray analysis revealed a dramatic up-regulation of gadd45 alpha a. Multiple assays indicate that mll inhibited gadd45 alpha a expression and that overexpression of gadd45 alpha a mRNA led to a phenotype similar to the one seen in the mll morphants. Taken together, these findings demonstrate that zebrafish mll plays essential roles in hematopoiesis and that gadd45 alpha a may serve as a potential downstream target for mediating the function of mll in hematopoiesis.
KeywordProto-oncoprotein Mll Hox Genes Acute Leukemias Stem-cell Definitive Hematopoiesis Drosophila-trithorax 11q23 Translocations Dna Demethylation Mutant Mice Histone
Department[Wan, XY; Hu, B; Liu, JX; Feng, X; Xiao, WH] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B]
Indexed BySCI
Language英语
WOS IDWOS:000294968800053
Funding OrganizationNational Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B] ; National Science Foundation of China[30971667, 31071212, 91019008, 20890113]; National Transgene Project[2009ZX08010-021B]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16351
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorXiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; w-xiao@ihb.ac.cn
Recommended Citation
GB/T 7714
Wan, Xiaoyang,Hu, Bo,Liu, Jing-xia,et al. Zebrafish mll Gene Is Essential for Hematopoiesis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(38):33345-33357.
APA Wan, Xiaoyang.,Hu, Bo.,Liu, Jing-xia.,Feng, Xi.,Xiao, Wuhan.,...&w-xiao@ihb.ac.cn.(2011).Zebrafish mll Gene Is Essential for Hematopoiesis.JOURNAL OF BIOLOGICAL CHEMISTRY,286(38),33345-33357.
MLA Wan, Xiaoyang,et al."Zebrafish mll Gene Is Essential for Hematopoiesis".JOURNAL OF BIOLOGICAL CHEMISTRY 286.38(2011):33345-33357.
Files in This Item:
File Name/Size DocType Version Access License
Zebrafish mll Gene I(4038KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wan, Xiaoyang]'s Articles
[Hu, Bo]'s Articles
[Liu, Jing-xia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wan, Xiaoyang]'s Articles
[Hu, Bo]'s Articles
[Liu, Jing-xia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wan, Xiaoyang]'s Articles
[Hu, Bo]'s Articles
[Liu, Jing-xia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Zebrafish mll Gene Is Essential for Hematopoiesis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.