IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site
Ke, Fei; He, Li-Bo; Pei, Chao; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; zhangqy@ihb.ac.cn
2011
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume12Pages:-
AbstractBackground: A virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. Therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further understanding of the taxonomic distribution of aquareovirus species and the molecular mechanism of its pathogenesis. Results: The full-length genome sequences of SMReV were determined. It comprises eleven dsRNA segments covering 24,042 base pairs and has the largest S4 genome segment in the sequenced aquareoviruses. Sequence analysis showed that all of the segments contained six conserved nucleotides at the 5' end and five conserved nucleotides at the 3' end (5'-GUUUUA - UCAUC-3'). The encoded amino acid sequences share the highest sequence identities with the respective proteins of aquareoviruses in species group Aquareovirus A. Phylogenetic analysis based on the major outer capsid protein VP7 and RNA-dependent RNA polymerase were performed. Members in Aquareovirus were clustered in two groups, one from fresh water fish and the other from marine fish. Furthermore, a fusion associated small transmembrane (FAST) protein NS22, which is translated from a non-AUG start site, was identified in the S7 segment. Conclusions: This study has provided the complete genome sequence of a novel isolated aquareovirus from marine fish. Amino acids comparison and phylogenetic analysis suggested that SMReV was a new aquareovirus in the species group Aquareovirus A. Phylogenetic analysis among aquareoviruses revealed that VP7 could be used as a reference to divide the aquareovirus from hosts in fresh water or marine. In addition, a FAST protein with a non-AUG start site was identified, which partially contributed to the cytopathic effect caused by the virus infection. These results provide new insights into the virus-host and virus-environment interactions.
KeywordGrass Carp Reovirus Family Reoviridae Genus Aquareovirus Amino-terminus Messenger-rna Sigma-ns Mu-ns Virus Sequence Initiation
Department[Ke, F; He, LB; Pei, C; Zhang, QY] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01]
Indexed BySCI
Language英语
WOS IDWOS:000292682100001
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01] ; National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01]
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16327
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; zhangqy@ihb.ac.cn
Recommended Citation
GB/T 7714
Ke, Fei,He, Li-Bo,Pei, Chao,et al. Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site[J]. BMC GENOMICS,2011,12:-.
APA Ke, Fei,He, Li-Bo,Pei, Chao,Zhang, Qi-Ya,Zhang, QY ,&zhangqy@ihb.ac.cn.(2011).Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site.BMC GENOMICS,12,-.
MLA Ke, Fei,et al."Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site".BMC GENOMICS 12(2011):-.
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