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学科主题: Biotechnology & Applied Microbiology; Genetics & Heredity
题名: Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site
作者: Ke, Fei ; He, Li-Bo ; Pei, Chao ; Zhang, Qi-Ya
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; zhangqy@ihb.ac.cn
关键词: GRASS CARP REOVIRUS ; FAMILY REOVIRIDAE ; GENUS AQUAREOVIRUS ; AMINO-TERMINUS ; MESSENGER-RNA ; SIGMA-NS ; MU-NS ; VIRUS ; SEQUENCE ; INITIATION
刊名: BMC GENOMICS
发表日期: 2011
卷: 12, 页:-
收录类别: SCI
部门归属: [Ke, F; He, LB; Pei, C; Zhang, QY] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
资助者: National Major Basic Research Program[2009CB118704, 2010CB126300]; National Natural Science Foundation of China[30871938, 31072239]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; Special Fund for Agro-scientific Research in the Public Interest[200803013]; State Key Laboratory of Freshwater Ecology and Biotechnology[O85B16-1-F01]
摘要: Background: A virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. Therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further understanding of the taxonomic distribution of aquareovirus species and the molecular mechanism of its pathogenesis. Results: The full-length genome sequences of SMReV were determined. It comprises eleven dsRNA segments covering 24,042 base pairs and has the largest S4 genome segment in the sequenced aquareoviruses. Sequence analysis showed that all of the segments contained six conserved nucleotides at the 5' end and five conserved nucleotides at the 3' end (5'-GUUUUA - UCAUC-3'). The encoded amino acid sequences share the highest sequence identities with the respective proteins of aquareoviruses in species group Aquareovirus A. Phylogenetic analysis based on the major outer capsid protein VP7 and RNA-dependent RNA polymerase were performed. Members in Aquareovirus were clustered in two groups, one from fresh water fish and the other from marine fish. Furthermore, a fusion associated small transmembrane (FAST) protein NS22, which is translated from a non-AUG start site, was identified in the S7 segment. Conclusions: This study has provided the complete genome sequence of a novel isolated aquareovirus from marine fish. Amino acids comparison and phylogenetic analysis suggested that SMReV was a new aquareovirus in the species group Aquareovirus A. Phylogenetic analysis among aquareoviruses revealed that VP7 could be used as a reference to divide the aquareovirus from hosts in fresh water or marine. In addition, a FAST protein with a non-AUG start site was identified, which partially contributed to the cytopathic effect caused by the virus infection. These results provide new insights into the virus-host and virus-environment interactions.
语种: 英语
WOS记录号: WOS:000292682100001
ISSN号: 1471-2164
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16327
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Recommended Citation:
Ke, Fei; He, Li-Bo; Pei, Chao; Zhang, Qi-Ya.Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site,BMC GENOMICS,2011,12():-
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文件名: Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site.pdf
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