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Isolation and Identification of a Novel Inducible Antibacterial Peptide from the Skin Mucus of Japanese Eel, Anguilla japonica
Liang, Ying; Guan, Ruizhang; Huang, Wenshu; Xu, Tongling; Guan, RZ (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; rzguan@jmu.edu.cn
2011
Source PublicationPROTEIN JOURNAL
ISSN1572-3887
Volume30Issue:6Pages:413-421
AbstractIn this study, acetone extracts and acidic extracts were prepared from skin mucus, gill, kidney, liver and spleen of the Japanese eel, Anguilla japonica, and they exhibited different levels of antibacterial activities against three strains of Gram-negative bacteria, Edwardsiella tarda, Aeromonas hydrophila, Aeromonas sp. and one Gram-positive bacterium Micrococcus leteus. The mucus was chosen as the source of antibacterial peptide for further purification of antibacterial peptides. Following the intraperitoneal injection of A. hydrophila, one of the main pathogenic bacteria of Japanese eel and many other fish, a peptide was purified from acetic acid extraction of the skin mucus, by using cationic exchange liquid chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). The isolated antibacterial peptide, named as AJN-10, exhibited antibacterial activity against A. hydrophila. The AJN-10 is a heat-tolerant and hydrophilic peptide. The molecular weight of this peptide is 6,044.28 Da, as determined by matrix-assisted laser desorption ionisation time of flight mass spectrometry. The 20 N-terminal amino acid sequences were clarified by Edman degradation, and based on results of homology search by BLAST analysis of the 20 N-terminal sequences, the AJN-10 showed little similarity to other proteins in databases.
KeywordAntibacterial Peptide Skin Mucus Anguilla Japonica Aeromons Hydrophila
Department[Liang, Y; Guan, RZ] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liang, Y; Guan, RZ; Huang, WS] Jimei Univ, Coll Fisheries, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 361021, Peoples R China; [Liang, Y] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationSpecial Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043]
Indexed BySCI
Language英语
WOS IDWOS:000293462400006
Funding OrganizationSpecial Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043] ; Special Scientific Research Fund of Agriculture Public Welfare Profession of China[nyhyzx07-043]
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Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16323
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuan, RZ (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; rzguan@jmu.edu.cn
Recommended Citation
GB/T 7714
Liang, Ying,Guan, Ruizhang,Huang, Wenshu,et al. Isolation and Identification of a Novel Inducible Antibacterial Peptide from the Skin Mucus of Japanese Eel, Anguilla japonica[J]. PROTEIN JOURNAL,2011,30(6):413-421.
APA Liang, Ying,Guan, Ruizhang,Huang, Wenshu,Xu, Tongling,Guan, RZ ,&rzguan@jmu.edu.cn.(2011).Isolation and Identification of a Novel Inducible Antibacterial Peptide from the Skin Mucus of Japanese Eel, Anguilla japonica.PROTEIN JOURNAL,30(6),413-421.
MLA Liang, Ying,et al."Isolation and Identification of a Novel Inducible Antibacterial Peptide from the Skin Mucus of Japanese Eel, Anguilla japonica".PROTEIN JOURNAL 30.6(2011):413-421.
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