IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis
Chuang-Ju, Li; Qi-Wei, Wei; Xi-Hua, Chen; Li, Zhou; Hong, Cao; Fang, Gan; Jian-Fang, Gui; Xi-Hua, C (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr China, Jinzhou 434000, Peoples R China; chenxh@yfi.ac.cn
2011
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume37Issue:3Pages:471-484
AbstractChinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288, and 1,290 bp in length, respectively, and they could be translated into proteins with 440, 394, and 398 amino acids, respectively. High level of amino acids sequence identity was seen between AsZP3.2 and AsZP3.3 (about 82%), but they share low identity with AsZP3.1 (26 and 23%, respectively). The AsZP3.1 has 42-50% amino acids sequence identity values with other fish and lower values with higher vertebrates (38%); AsZP3.2 and AsZP3.3 shared about 30-44% sequence identity with higher vertebrates and other fish. RT-PCR analysis indicated that AsZP3.1 displayed a wide tissue distribution at the mRNA levels including liver, kidney, spleen, heart, and ovary, but AsZP3.2 and AsZP3.3 mRNAs were expressed exclusively in the gonad. All three AsZP3 mRNAs were not detected during embryogenesis and early larval development; furthermore, they were not detected in the gonads of 1- and 2-year-old Chinese sturgeons. All three AsZP3 mRNAs were detected in the testes of 3-year-old males and in the ovaries of 4- and 5-year-old female Chinese sturgeons.
KeywordChinese Sturgeon Zona Pellucida Protein 3 Gene Expression Rt-pcr
Department[Chuang-Ju, L; Qi-Wei, W; Xi-Hua, C; Fang, G] Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr China, Jinzhou 434000, Peoples R China; [Li, Z; Jian-Fang, G] Chinese Acad Sci, Wuhan Ctr Dev Biol, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chuang-Ju, L; Qi-Wei, W; Xi-Hua, C] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China; [Hong, C] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007]
Indexed BySCI
Language英语
WOS IDWOS:000293295800013
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16319
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorXi-Hua, C (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr China, Jinzhou 434000, Peoples R China; chenxh@yfi.ac.cn
Recommended Citation
GB/T 7714
Chuang-Ju, Li,Qi-Wei, Wei,Xi-Hua, Chen,et al. Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2011,37(3):471-484.
APA Chuang-Ju, Li.,Qi-Wei, Wei.,Xi-Hua, Chen.,Li, Zhou.,Hong, Cao.,...&chenxh@yfi.ac.cn.(2011).Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis.FISH PHYSIOLOGY AND BIOCHEMISTRY,37(3),471-484.
MLA Chuang-Ju, Li,et al."Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis".FISH PHYSIOLOGY AND BIOCHEMISTRY 37.3(2011):471-484.
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