IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis
Li, Xuemei; Yu, Yuhe; Xie, Shouqi; Yan, Qingyun; Chen, Yuhang; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2011
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
ISSN0893-8849
Volume42Issue:4Pages:539-548
AbstractA 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan-containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class gamma-Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas-specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp.
KeywordTrout Oncorhynchus-mykiss 16s Ribosomal-rna Early-life Stages Sole Solea-Solea Fresh-water Fish Phylogenetic Analysis Molecular-identification Gastrointestinal-tract Microbial Community Cyprinus-carpio
Department[Li, XM; Yu, YH; Yan, QY] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Li, XM; Chen, YH] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xie, SQ; Chen, YH] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries
Funding OrganizationMajor State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205]
Indexed BySCI
Language英语
WOS IDWOS:000293351200008
Funding OrganizationMajor State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16281
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Xuemei,Yu, Yuhe,Xie, Shouqi,et al. Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2011,42(4):539-548.
APA Li, Xuemei,Yu, Yuhe,Xie, Shouqi,Yan, Qingyun,Chen, Yuhang,&Yu, YH .(2011).Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,42(4),539-548.
MLA Li, Xuemei,et al."Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 42.4(2011):539-548.
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