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学科主题: Fisheries
题名: Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis
作者: Li, Xuemei1, 2; Yu, Yuhe1; Xie, Shouqi3; Yan, Qingyun1; Chen, Yuhang2, 3
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
关键词: TROUT ONCORHYNCHUS-MYKISS ; 16S RIBOSOMAL-RNA ; EARLY-LIFE STAGES ; SOLE SOLEA-SOLEA ; FRESH-WATER FISH ; PHYLOGENETIC ANALYSIS ; MOLECULAR-IDENTIFICATION ; GASTROINTESTINAL-TRACT ; MICROBIAL COMMUNITY ; CYPRINUS-CARPIO
刊名: JOURNAL OF THE WORLD AQUACULTURE SOCIETY
发表日期: 2011-08-01
DOI: 10.1111/j.1749-7345.2011.00496.x
卷: 42, 期:4, 页:539-548
收录类别: SCI
文章类型: Article
部门归属: [Li, XM; Yu, YH; Yan, QY] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Li, XM; Chen, YH] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xie, SQ; Chen, YH] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Major State Basic Research Development Program of China[2009CB118705, 2007CB109205]
类目[WOS]: Fisheries
研究领域[WOS]: Fisheries
摘要: A 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan-containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class gamma-Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas-specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp.
英文摘要: A 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan-containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class gamma-Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas-specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; 16S RIBOSOMAL-RNA ; EARLY-LIFE STAGES ; SOLE SOLEA-SOLEA ; FRESH-WATER FISH ; PHYLOGENETIC ANALYSIS ; MOLECULAR-IDENTIFICATION ; GASTROINTESTINAL-TRACT ; MICROBIAL COMMUNITY ; CYPRINUS-CARPIO
语种: 英语
WOS记录号: WOS:000293351200008
ISSN号: 0893-8849
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16281
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Xuemei; Yu, Yuhe; Xie, Shouqi; Yan, Qingyun; Chen, Yuhang.Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis,JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2011,42(4):539-548
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