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Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts
Lan, Shubin; Wu, Li; Zhang, Delu; Hu, Chunxiang; Liu, Yongding; Hu, CX, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; cxhu@ihb.ac.cn
2011
Source PublicationSOIL BIOLOGY & BIOCHEMISTRY
ISSN0038-0717
Volume43Issue:4Pages:857-861
AbstractIn order to estimate the biomass of photoautotrophic organisms in biological soil crusts (BSCs), based on the extraction and determination of phytoplanktonic chlorophyll-a (Chl-a) in aquatic ecosystems, this paper comparatively studied the Chl-a extraction efficiencies in ethanol, acetone, N. N -dimethyl -formamide (DMF) and dimethyl sulphoxide (DMSO) from algae, lichen, and moss crusts, analyzed the effects of dominant organisms or development degree of BSCs, mass of sample, and soil characteristics on the extraction efficiency. The results showed that the extraction efficiencies of different organic solvents were significantly different, and such efficiencies declined with the increase of mass of sample. DMSO resulted in the greatest extraction efficiency, but was not suitable for lichen crusts; DMF strongly underestimated the Chl-a content of man-made crusts relative to the other extractants, and was particularly affected by interfering pigments from the well-developed crusts. Generally, the extraction efficiency of ethanol was greater than that of acetone, and ethanol method showed the greatest stability compared with other 3 solvents. In addition, Chia content showed an increasing trend with the succession of BSCs when it was expressed on an areal basis. On the basis of this and related experiments we suggest Chl-a content of BSCs be extracted by ethanol and expressed on an areal basis. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordPhotoautotrophic Biomass Extraction Efficiency Mass Of Sample Development And Succession Soil Characteristics
Department[Lan, Shubin; Wu, Li; Zhang, Delu; Hu, Chunxiang; Liu, Yongding] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Lan, Shubin; Wu, Li] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Zhang, Delu] Wuhan Univ Technol, Dept Biol Sci & Biotechnol, Wuhan 430070, Peoples R China
Subject AreaSoil Science
Funding OrganizationNational Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization
Indexed BySCI
Language英语
WOS IDWOS:000288571700019
Funding OrganizationNational Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization ; National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15686
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, CX, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; cxhu@ihb.ac.cn
Recommended Citation
GB/T 7714
Lan, Shubin,Wu, Li,Zhang, Delu,et al. Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts[J]. SOIL BIOLOGY & BIOCHEMISTRY,2011,43(4):857-861.
APA Lan, Shubin.,Wu, Li.,Zhang, Delu.,Hu, Chunxiang.,Liu, Yongding.,...&cxhu@ihb.ac.cn.(2011).Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts.SOIL BIOLOGY & BIOCHEMISTRY,43(4),857-861.
MLA Lan, Shubin,et al."Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts".SOIL BIOLOGY & BIOCHEMISTRY 43.4(2011):857-861.
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