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Expression profiles of carp IRF-3/-7 correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK1, four pivotal molecules in RIG-I signaling pathway
Feng, Hong; Liu, Hong; Kong, Renqiu; Wang, Lu; Wang, Yaping; Hu, Wei; Guo, Qionglin; Kong, RQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; kongr@ihb.ac.cn; qlguo@ihb.ac.cn
2011
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume30Issue:40638Pages:1159-1169
AbstractThe cytoplasmic helicase protein RIG-I (retinoic acid-inducible gene I) and downstream signaling molecules, MAVS (mitochondrial antiviral signaling protein), TRAF3 (TNF-receptor-associated factor 3) and TBK1 (TANK-binding kinase 1), have significant roles in the recognition of cytoplasmic 5'-triphosphate ssRNA and short dsRNA, and phosphorylation of IRF-3 (interferon regulatory factor 3) and IRF-7 which is responsible for the induction of type I interferons (IFN). In the present study, the full-length cDNAs of RIG-I, MAVS, TRAF3 and TBK1 were cloned and identified in common carp (Cyprinus carpio L). The deduced protein of carp RIG-I is of 946 aa (amino acids), consisting of two CARDs (caspase-recruitment domain), a DEXDc (DExD/H box-containing domain), a HELICc (helicase superfamily c-terminal domain) and a RD (regulatory domain). Carp MAVS is of 585 aa, containing a CARD, a proline-rich region and a TM (transmembrane domain). Carp TRAF3 encodes a protein of 573 aa, including a RING (really interesting new gene), two TRAF-type zinc fingers, a coiled coil and a MATH-TRAF3 (meprin and TRAF homology) domain. Carp TBK1 is of 727 aa and contains a S_TKc domain (Serine/Threonine protein kinases, catalytic domain). Carp RIG-I, MAVS, TRAF3 and TBK1 mRNAs are ubiquitously expressed in all tissues examined. In response to SVCV infection, carp RIG-I and MAVS mRNAs were up-regulated at different levels in spleen, head kidney and intestine tissues at different time points. Similarly, both carp IRF-3 and IRF-7 mRNAs were significantly up-regulated in the detected tissues. Especially in intestine, the IRF-3 and IRF-7 mRNAs of carp increased and reached 25.3-fold (at 3 dpi) and 224.7-fold (at 5 dpi). Noteworthily, a significant growth of carp TRAF3 and TBK1 mRNA was also mainly found in intestine (7.0-fold and 11.3-fold at 5 dpi, respectively). These data implied that the expression profiles of IRF-3/-7 mRNAs in carp correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK, and carp RIG-I and MAVS may be involved in antiviral responses through the RIG-I viral recognition signaling pathway in a TRAF3/TBK1-dependent manner. (C) 2011 Elsevier Ltd. All rights reserved.
KeywordRig-i Mavs Traf3 Tbk1 Common Carp
Department[Feng, Hong; Kong, Renqiu; Wang, Lu; Wang, Yaping; Hu, Wei; Guo, Qionglin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Liu, Hong] Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China; [Feng, Hong; Wang, Lu] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05]
Indexed BySCI
WOS IDWOS:000289969000020
Funding OrganizationNational Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB05]
Citation statistics
Cited Times:71[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15682
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorKong, RQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; kongr@ihb.ac.cn; qlguo@ihb.ac.cn
Recommended Citation
GB/T 7714
Feng, Hong,Liu, Hong,Kong, Renqiu,et al. Expression profiles of carp IRF-3/-7 correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK1, four pivotal molecules in RIG-I signaling pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(40638):1159-1169.
APA Feng, Hong.,Liu, Hong.,Kong, Renqiu.,Wang, Lu.,Wang, Yaping.,...&qlguo@ihb.ac.cn.(2011).Expression profiles of carp IRF-3/-7 correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK1, four pivotal molecules in RIG-I signaling pathway.FISH & SHELLFISH IMMUNOLOGY,30(40638),1159-1169.
MLA Feng, Hong,et al."Expression profiles of carp IRF-3/-7 correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK1, four pivotal molecules in RIG-I signaling pathway".FISH & SHELLFISH IMMUNOLOGY 30.40638(2011):1159-1169.
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