IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
MRP proteins as potential mediators of heavy metal resistance in zebrafish cells
Long, Yong; Li, Qing; Wang, Youhui; Cui, Zongbin; Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China; zbcui@ihb.ac.cn
2011
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
Volume153Issue:3Pages:310-317
AbstractAcquired resistance of mammalian cells to heavy metals is closely relevant to enhanced expression of several multidrug resistance-associated proteins (MRP), but it remains unclear whether MRP proteins confer resistance to heavy metals in zebrafish. In this study, we obtained zebrafish (Danio rerio) fibroblast-like ZF4 cells with resistance to toxic heavy metals after chronic cadmium exposure and selection for 6 months. These cadmium-resistant cells (ZF4-Cd) were maintained in 5 mu M cadmium and displayed cross-resistance to cadmium, mercury, arsenite and arsenate. ZF4-Cd cells remained the resistance to heavy metals after protracted culture in cadmium-free medium. In comparison with ZF4-WT cells, ZF4-Cd cells exhibited accelerated rate of cadmium excretion, enhanced activity of MRP-like transport, elevated expression of abcc2, abcc4 and mt2 genes, and increased content of cellular GSH. Inhibition of MRP-like transport activity, GSH biosynthesis and GST activity significantly attenuated the resistance of ZF4-Cd cells to heavy metals. The results indicate that some of MRP transporters are involved in the efflux of heavy metals conjugated with cellular GSH and thus play crucial roles in heavy metal detoxification of zebrafish cells. (C) 2010 Elsevier Inc. All rights reserved.
KeywordZebrafish Mrp Gsh Gst Heavy Metal Detoxification
Department[Long, Yong; Li, Qing; Cui, Zongbin] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wang, Youhui] Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Zhejiang, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
Funding OrganizationNational Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442]
Indexed BySCI
Language英语
WOS IDWOS:000288298700006
Funding OrganizationNational Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442]
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15678
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China; zbcui@ihb.ac.cn
Recommended Citation
GB/T 7714
Long, Yong,Li, Qing,Wang, Youhui,et al. MRP proteins as potential mediators of heavy metal resistance in zebrafish cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2011,153(3):310-317.
APA Long, Yong,Li, Qing,Wang, Youhui,Cui, Zongbin,Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China,&zbcui@ihb.ac.cn.(2011).MRP proteins as potential mediators of heavy metal resistance in zebrafish cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,153(3),310-317.
MLA Long, Yong,et al."MRP proteins as potential mediators of heavy metal resistance in zebrafish cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 153.3(2011):310-317.
Files in This Item:
File Name/Size DocType Version Access License
MRP proteins as pote(2622KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Long, Yong]'s Articles
[Li, Qing]'s Articles
[Wang, Youhui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Long, Yong]'s Articles
[Li, Qing]'s Articles
[Wang, Youhui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Long, Yong]'s Articles
[Li, Qing]'s Articles
[Wang, Youhui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: MRP proteins as potential mediators of heavy metal resistance in zebrafish cells.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.