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学科主题: Genetics & Heredity
题名: Molecular analysis and functions of p53R2 in zebrafish
作者: Shang, Hanqiao1, 2; Li, Qing1; Feng, Guohui1, 2; Cui, Zongbin1
通讯作者: Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organism, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China ; zbcui@ihb.ac.cn
关键词: Zebrafish ; p53R2 ; DNA damage ; DNA synthesis ; Genotoxicity
刊名: GENE
发表日期: 2011-04-01
DOI: 10.1016/j.gene.2010.12.008
卷: 475, 期:1, 页:30-38
收录类别: SCI
文章类型: Article
部门归属: [Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China; [Shang, Hanqiao; Feng, Guohui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30671602, 30871442, 30623001]
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
摘要: p53R2 is a newly identified small subunit of ribonucleotide reductase and plays a pivotal role in the supply of dNTPs for genomic DNA repair and mitochondrial DNA synthesis, but little is known about its functions in zebrafish. Herein, we obtained the cDNA of zebrafish p53R2 that shares 72.8% and 72.5% amino acid identities with human p53R2 and zebrafish R2, respectively. Residues crucial for enzymatic activity are highly conserved among p53R2 proteins from different species. p53R2 in zebrafish was maternally expressed, its transcripts were detected in developing embryos and all adult tissues examined. A 250-bp minimal promoter upstream of the translational initiation site was identified to drive basal expression of p53R2 in a p53-independent manner. Expression of p53R2 was induced by DNA-damaging reagents CPT or MMS, but suppressed by p53-knockdown in zebrafish embryos. Moreover, p53R2 was mainly distributed in the cytoplasm of cells under normal condition and upon DNA damage. Furthermore, overexpression of p53R2 attenuated apoptosis of embryonic cells caused by CPT or MMS treatment and protected developing embryos from death. Therefore, functions of p53R2 in zebrafish are closely associated with its activity in DNA repair and synthesis. (C) 2010 Elsevier B.V. All rights reserved.
英文摘要: p53R2 is a newly identified small subunit of ribonucleotide reductase and plays a pivotal role in the supply of dNTPs for genomic DNA repair and mitochondrial DNA synthesis, but little is known about its functions in zebrafish. Herein, we obtained the cDNA of zebrafish p53R2 that shares 72.8% and 72.5% amino acid identities with human p53R2 and zebrafish R2, respectively. Residues crucial for enzymatic activity are highly conserved among p53R2 proteins from different species. p53R2 in zebrafish was maternally expressed, its transcripts were detected in developing embryos and all adult tissues examined. A 250-bp minimal promoter upstream of the translational initiation site was identified to drive basal expression of p53R2 in a p53-independent manner. Expression of p53R2 was induced by DNA-damaging reagents CPT or MMS, but suppressed by p53-knockdown in zebrafish embryos. Moreover, p53R2 was mainly distributed in the cytoplasm of cells under normal condition and upon DNA damage. Furthermore, overexpression of p53R2 attenuated apoptosis of embryonic cells caused by CPT or MMS treatment and protected developing embryos from death. Therefore, functions of p53R2 in zebrafish are closely associated with its activity in DNA repair and synthesis. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: MOUSE RIBONUCLEOTIDE REDUCTASE ; MITOCHONDRIAL-DNA DEPLETION ; RADICAL TRANSFER PATHWAY ; CELL-CYCLE CHECKPOINT ; GENE-EXPRESSION ; DRUG DISCOVERY ; RENAL-FAILURE ; SMALL-SUBUNIT ; PROTEIN R2 ; DNTP POOLS
语种: 英语
WOS记录号: WOS:000288936100004
ISSN号: 0378-1119
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15674
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin.Molecular analysis and functions of p53R2 in zebrafish,GENE,2011,475(1):30-38
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