Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION | |
Hu, Yunfei; Zhang, Xinxin; Shi, Yunming; Zhou, Yanfeng; Zhang, Wei; Su, Xiao-Dong; Xia, Bin; Zhao, Jindong; Jin, Changwen; Zhao, JD, Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China; jzhao@pku.edu.cn; changwen@pku.edu.cn | |
2011 | |
Source Publication | JOURNAL OF BIOLOGICAL CHEMISTRY
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ISSN | 0021-9258 |
Volume | 286Issue:14Pages:- |
Abstract | Ca2+-binding proteins play pivotal roles in both eukaryotic and prokaryotic cells. CcbP from cyanobacterium Anabaena sp. strain PCC 7120 is a major Ca2+-binding protein involved in heterocyst differentiation, a process that forms specialized nitrogen-fixing cells. The three-dimensional structures of both Ca2+-free and Ca2+-bound forms of CcbP are essential for elucidating the Ca2+-signaling mechanism. However, CcbP shares low sequence identity with proteins of known structures, and its Ca2+-binding sites remain unknown. Here, we report the solution structures of CcbP in both Ca2+-free and Ca2+-bound forms determined by nuclear magnetic resonance spectroscopy. CcbP adopts an overall new fold and contains two Ca2+-binding sites with distinct Ca2+-binding abilities. Mutation of Asp(38) at the stronger Ca2+-binding site of CcbP abolished its ability to regulate heterocyst formation in vivo. Surprisingly, the beta-barrel subdomain of CcbP, which does not participate in Ca2+-binding, topologically resembles the Src homology 3 (SH3) domain and might act as a protein-protein interaction module. Our results provide the structural basis of the unique Ca2+ signaling mechanism during heterocyst differentiation. |
Keyword | Diphtheria-toxin Repressor Torsion Angle Dynamics Ef-hand Proteins Nmr Experiments Noe Assignment Sh3 Domains Homology Src Cyanobacteria Programs |
Department | [Hu, Yunfei; Zhang, Xinxin; Xia, Bin; Zhao, Jindong; Jin, Changwen] Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China; [Shi, Yunming; Zhang, Wei; Zhao, Jindong; Jin, Changwen] Peking Univ, State Key Lab Plant & Prot Engn, Beijing 100871, Peoples R China; [Xia, Bin; Zhao, Jindong] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China; [Xia, Bin; Jin, Changwen] Peking Univ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China; [Zhao, Jindong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
Subject Area | Biochemistry & Molecular Biology |
Funding Organization | National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] |
Indexed By | SCI |
Language | 英语 |
WOS ID | WOS:000289077500052 |
Funding Organization | National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] ; National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323] |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/15672 |
Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
Corresponding Author | Zhao, JD, Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China; jzhao@pku.edu.cn; changwen@pku.edu.cn |
Recommended Citation GB/T 7714 | Hu, Yunfei,Zhang, Xinxin,Shi, Yunming,et al. Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(14):-. |
APA | Hu, Yunfei.,Zhang, Xinxin.,Shi, Yunming.,Zhou, Yanfeng.,Zhang, Wei.,...&changwen@pku.edu.cn.(2011).Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION.JOURNAL OF BIOLOGICAL CHEMISTRY,286(14),-. |
MLA | Hu, Yunfei,et al."Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION".JOURNAL OF BIOLOGICAL CHEMISTRY 286.14(2011):-. |
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