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学科主题: Biochemistry & Molecular Biology
题名: Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION
作者: Hu, Yunfei1; Zhang, Xinxin1; Shi, Yunming2; Zhou, Yanfeng; Zhang, Wei2; Su, Xiao-Dong; Xia, Bin1, 3, 4; Zhao, Jindong1, 2, 3, 5; Jin, Changwen1, 2, 4
通讯作者: Zhao, JD, Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China ; jzhao@pku.edu.cn ; changwen@pku.edu.cn
关键词: DIPHTHERIA-TOXIN REPRESSOR ; TORSION ANGLE DYNAMICS ; EF-HAND PROTEINS ; NMR EXPERIMENTS ; NOE ASSIGNMENT ; SH3 DOMAINS ; HOMOLOGY ; SRC ; CYANOBACTERIA ; PROGRAMS
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2011-04-08
DOI: 10.1074/jbc.M110.201186
卷: 286, 期:14, 页:-
收录类别: SCI
文章类型: Article
部门归属: [Hu, Yunfei; Zhang, Xinxin; Xia, Bin; Zhao, Jindong; Jin, Changwen] Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China; [Shi, Yunming; Zhang, Wei; Zhao, Jindong; Jin, Changwen] Peking Univ, State Key Lab Plant & Prot Engn, Beijing 100871, Peoples R China; [Xia, Bin; Zhao, Jindong] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China; [Xia, Bin; Jin, Changwen] Peking Univ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China; [Zhao, Jindong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation [30325010, 30230040]; Ministry of Science and Technology of China [2006CB910203, 2006AA02A323]
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Ca2+-binding proteins play pivotal roles in both eukaryotic and prokaryotic cells. CcbP from cyanobacterium Anabaena sp. strain PCC 7120 is a major Ca2+-binding protein involved in heterocyst differentiation, a process that forms specialized nitrogen-fixing cells. The three-dimensional structures of both Ca2+-free and Ca2+-bound forms of CcbP are essential for elucidating the Ca2+-signaling mechanism. However, CcbP shares low sequence identity with proteins of known structures, and its Ca2+-binding sites remain unknown. Here, we report the solution structures of CcbP in both Ca2+-free and Ca2+-bound forms determined by nuclear magnetic resonance spectroscopy. CcbP adopts an overall new fold and contains two Ca2+-binding sites with distinct Ca2+-binding abilities. Mutation of Asp(38) at the stronger Ca2+-binding site of CcbP abolished its ability to regulate heterocyst formation in vivo. Surprisingly, the beta-barrel subdomain of CcbP, which does not participate in Ca2+-binding, topologically resembles the Src homology 3 (SH3) domain and might act as a protein-protein interaction module. Our results provide the structural basis of the unique Ca2+ signaling mechanism during heterocyst differentiation.
英文摘要: Ca2+-binding proteins play pivotal roles in both eukaryotic and prokaryotic cells. CcbP from cyanobacterium Anabaena sp. strain PCC 7120 is a major Ca2+-binding protein involved in heterocyst differentiation, a process that forms specialized nitrogen-fixing cells. The three-dimensional structures of both Ca2+-free and Ca2+-bound forms of CcbP are essential for elucidating the Ca2+-signaling mechanism. However, CcbP shares low sequence identity with proteins of known structures, and its Ca2+-binding sites remain unknown. Here, we report the solution structures of CcbP in both Ca2+-free and Ca2+-bound forms determined by nuclear magnetic resonance spectroscopy. CcbP adopts an overall new fold and contains two Ca2+-binding sites with distinct Ca2+-binding abilities. Mutation of Asp(38) at the stronger Ca2+-binding site of CcbP abolished its ability to regulate heterocyst formation in vivo. Surprisingly, the beta-barrel subdomain of CcbP, which does not participate in Ca2+-binding, topologically resembles the Src homology 3 (SH3) domain and might act as a protein-protein interaction module. Our results provide the structural basis of the unique Ca2+ signaling mechanism during heterocyst differentiation.
关键词[WOS]: DIPHTHERIA-TOXIN REPRESSOR ; TORSION ANGLE DYNAMICS ; EF-HAND PROTEINS ; NMR EXPERIMENTS ; NOE ASSIGNMENT ; SH3 DOMAINS ; HOMOLOGY ; SRC ; CYANOBACTERIA ; PROGRAMS
语种: 英语
WOS记录号: WOS:000289077500052
ISSN号: 0021-9258
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15672
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Peking Univ, Coll Life Sci, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China
2.Peking Univ, State Key Lab Plant & Prot Engn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
4.Peking Univ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Hu, Yunfei; Zhang, Xinxin; Shi, Yunming; Zhou, Yanfeng; Zhang, Wei; Su, Xiao-Dong; Xia, Bin; Zhao, Jindong; Jin, Changwen.Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION,JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(14):-
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文件名: Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION.pdf
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