IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents
Zhang, Min; Zhang, Chao; Li, Jia; Hanna, Michelle; Zhang, Xiaohua; Dai, Heping; Xiao, Wei; Xiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada; wei.xiao@usask.ca
2011
Source PublicationTOXICOLOGICAL SCIENCES
ISSN1096-6080
Volume120Issue:2Pages:310-321
AbstractDespite the great advances by using microorganism-based genotoxicity testing systems to assess environmental genotoxic compounds, most of them respond poorly, particularly to oxidative agents. In this study, we systematically examined the RNR3-lacZ reporter gene expression in Saccharomyces cerevisiae mutant strains defective in the protection against reactive oxygen species and found that only YAP1 deletion resulted in a significant enhancement in the detection of oxidative damage. To our surprise, YAP1 deletion also caused an increased cellular sensitivity to a variety of DNA damage. This altered sensitivity appears to be independent of oxidative damage because under conditions in which vitamin C treatment rescued oxidative damage, it failed to reverse the phenotypes caused by other types of DNA damage. Furthermore, although inactivation of cell permeability genes enhanced the RNR3-lacZ detection sensitivity particularly to large molecular weight compounds, their effects on small molecular oxidative agents are minimal. Taken together, this study helps to create a hypersensitive genotoxicity testing system to a broad range of DNA-damaging agents by deleting a single yeast gene.
KeywordRnr3-lacz Yeast Oxidative Compounds Yap1 Genotoxicity Test Sensitivity
Department[Li, Jia; Hanna, Michelle; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada; [Zhang, Min; Zhang, Chao; Zhang, Xiaohua; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Subject AreaToxicology
Funding OrganizationNatural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08]
Indexed BySCI
Language英语
WOS IDWOS:000288804800007
Funding OrganizationNatural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08] ; Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15670
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorXiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada; wei.xiao@usask.ca
Recommended Citation
GB/T 7714
Zhang, Min,Zhang, Chao,Li, Jia,et al. Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents[J]. TOXICOLOGICAL SCIENCES,2011,120(2):310-321.
APA Zhang, Min.,Zhang, Chao.,Li, Jia.,Hanna, Michelle.,Zhang, Xiaohua.,...&wei.xiao@usask.ca.(2011).Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents.TOXICOLOGICAL SCIENCES,120(2),310-321.
MLA Zhang, Min,et al."Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents".TOXICOLOGICAL SCIENCES 120.2(2011):310-321.
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