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学科主题: Toxicology
题名: Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents
作者: Zhang, Min ; Zhang, Chao ; Li, Jia ; Hanna, Michelle ; Zhang, Xiaohua ; Dai, Heping ; Xiao, Wei
通讯作者: Xiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada ; wei.xiao@usask.ca
关键词: RNR3-lacZ ; yeast ; oxidative compounds ; YAP1 ; genotoxicity test ; sensitivity
刊名: TOXICOLOGICAL SCIENCES
发表日期: 2011
卷: 120, 期:2, 页:310-321
收录类别: SCI
部门归属: [Li, Jia; Hanna, Michelle; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada; [Zhang, Min; Zhang, Chao; Zhang, Xiaohua; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
资助者: Natural Science Foundation of China [20977108]; Natural Sciences and Engineering Research Council of Canada [OPG0138338]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2010FB03, 2009FBZ08]
摘要: Despite the great advances by using microorganism-based genotoxicity testing systems to assess environmental genotoxic compounds, most of them respond poorly, particularly to oxidative agents. In this study, we systematically examined the RNR3-lacZ reporter gene expression in Saccharomyces cerevisiae mutant strains defective in the protection against reactive oxygen species and found that only YAP1 deletion resulted in a significant enhancement in the detection of oxidative damage. To our surprise, YAP1 deletion also caused an increased cellular sensitivity to a variety of DNA damage. This altered sensitivity appears to be independent of oxidative damage because under conditions in which vitamin C treatment rescued oxidative damage, it failed to reverse the phenotypes caused by other types of DNA damage. Furthermore, although inactivation of cell permeability genes enhanced the RNR3-lacZ detection sensitivity particularly to large molecular weight compounds, their effects on small molecular oxidative agents are minimal. Taken together, this study helps to create a hypersensitive genotoxicity testing system to a broad range of DNA-damaging agents by deleting a single yeast gene.
语种: 英语
WOS记录号: WOS:000288804800007
ISSN号: 1096-6080
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15670
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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Recommended Citation:
Zhang, Min; Zhang, Chao; Li, Jia; Hanna, Michelle; Zhang, Xiaohua; Dai, Heping; Xiao, Wei.Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents,TOXICOLOGICAL SCIENCES,2011,120(2):310-321
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文件名: Inactivation of YAP1 Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System to a Broad Range of DNA-Damaging Agents.pdf
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