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Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage
Xiong, Daowen1,2; Fang, Tao1; Yu, Linpeng1,2; Sima, Xiaofeng1,2; Zhu, Wentao1,2; Fang, T, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2011-03-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume409Issue:8Pages:1444-1452
AbstractThe acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO2 NPs (96-h LC50 of 124.5 mg/L) to zebrafish was greater than that of the bulk TiO2, which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn2+ solution were quite similar to each other (96-h LC50 of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs,center dot OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of center dot OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of center dot OH. In contrast, exposure to 5 mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50 mg/L TiO2 NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5 mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms. (C) 2011 Elsevier B.V. All rights reserved.; The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO2 NPs (96-h LC50 of 124.5 mg/L) to zebrafish was greater than that of the bulk TiO2, which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn2+ solution were quite similar to each other (96-h LC50 of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs,center dot OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of center dot OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of center dot OH. In contrast, exposure to 5 mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50 mg/L TiO2 NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5 mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms. (C) 2011 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordEco-toxicity Metal Oxide Nanoparticle Tio2 Zno Ros Oxidative Damage
Subject AreaEnvironmental Sciences
Department[Xiong, Daowen; Fang, Tao; Yu, Linpeng; Sima, Xiaofeng; Zhu, Wentao] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Xiong, Daowen; Yu, Linpeng; Sima, Xiaofeng; Zhu, Wentao] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1016/j.scitotenv.2011.01.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationKey Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004]
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS KeywordTITANIUM-DIOXIDE NANOPARTICLES ; PERSONAL CARE PRODUCTS ; DAPHNIA-MAGNA ; PSEUDOKIRCHNERIELLA-SUBCAPITATA ; PHOTOCATALYTIC DEGRADATION ; ULTRAFINE PARTICLES ; ONCORHYNCHUS-MYKISS ; CARBON NANOTUBES ; NANOSCALE TIO2 ; FULLERENE C-60
WOS IDWOS:000288634300010
Funding OrganizationKey Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004] ; Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004]
Citation statistics
Cited Times:266[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15654
Collection水环境工程研究中心
Corresponding AuthorFang, T, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Daowen,Fang, Tao,Yu, Linpeng,et al. Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2011,409(8):1444-1452.
APA Xiong, Daowen,Fang, Tao,Yu, Linpeng,Sima, Xiaofeng,Zhu, Wentao,&Fang, T, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2011).Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage.SCIENCE OF THE TOTAL ENVIRONMENT,409(8),1444-1452.
MLA Xiong, Daowen,et al."Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage".SCIENCE OF THE TOTAL ENVIRONMENT 409.8(2011):1444-1452.
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