| Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides | |
| Wei, You Chuan; Pan, Ting Shuang; Chang, Ming Xian; Huang, Bei; Xu, Zhen; Luo, Ting Rong; Nie, P.; Luo, TR, Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, 100 Daxue Rd, Nanning 530004, Guangxi Autonom, Peoples R China; tingrongluo@gxu.edu.cn; pinnie@ihb.ac.cn | |
| 2011 | |
| Source Publication | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| ISSN | 0165-2427 |
| Volume | 141Issue:40606Pages:173-182 |
| Abstract | The two Toll-like receptors, TLR1 and TLR2 were cloned from orange-spotted grouper, Epinephelus coioides, an important teleost fish in mariculture of Asia. The cDNA sequences of TLR1 and TLR2 are 3195 and 3439 bp long, with an open reading frame (ORF) of 2406 and 2466 bp, encoding 801 and 821 amino acids, respectively. The TLR family motifs, i.e. leucine-rich repeat (LRR) domains and Toll/interleukin (IL)-1 receptor (TIR) domains are conserved in the TLR1 and TLR2, with nine and ten leucine-rich repeat (LRR) domains, and with one TIR domain, respectively. The TLR1 and TLR2 had a constitutive expression in examined organs/tissues of nave orange-spotted grouper, and an increased expression of TLR1 and TLR2 at mRNA level was observed in immune organs such as in spleen of LPS and Poly(I:C) stimulated fish. An increased expression of TLR1 and TLR2 was also recorded in immune organs of the fish injected with the bacterial pathogen, Vibrio alginolyticus. Similarly, a significant rise in the expression of MyD88, an adaptor molecule which forms signalling complex with intracellular TIR domain, thus leading to the production of pro-inflammatory cytokines, such as IL-1 beta, was also observed in the LPS- and Poly(I:C)-stimulated, and V. alginolyticus-infected fish, indicating the possible role of TLR1 and TLR2 in the MyD88 signalling pathway. However, the mechanism involved in the increased expression of TLR1 and TLR2 following LPS and Poly(I:C) stimulation is at present unknown in fish, and further research should be carried out to identify ligands of fish TLR1 and TLR2 in order to understand the function of these receptors. (C) 2011 Elsevier B.V. All rights reserved. |
| Keyword | Toll-like Receptor Tlr 1 Tlr2 Expression Orange-spotted Grouper |
| Department | [Wei, You Chuan; Luo, Ting Rong] Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Nanning 530004, Guangxi Autonom, Peoples R China; [Wei, You Chuan; Pan, Ting Shuang; Chang, Ming Xian; Huang, Bei; Xu, Zhen; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Pan, Ting Shuang] Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Anhui, Peoples R China |
| Subject Area | Immunology ; Veterinary Sciences |
| Funding Organization | National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000291139200001 |
| Funding Organization | National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/15636 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Luo, TR, Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, 100 Daxue Rd, Nanning 530004, Guangxi Autonom, Peoples R China; tingrongluo@gxu.edu.cn; pinnie@ihb.ac.cn |
| Recommended Citation GB/T 7714 | Wei, You Chuan,Pan, Ting Shuang,Chang, Ming Xian,et al. Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2011,141(40606):173-182. |
| APA | Wei, You Chuan.,Pan, Ting Shuang.,Chang, Ming Xian.,Huang, Bei.,Xu, Zhen.,...&pinnie@ihb.ac.cn.(2011).Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,141(40606),173-182. |
| MLA | Wei, You Chuan,et al."Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 141.40606(2011):173-182. |
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