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学科主题: Immunology; Veterinary Sciences
题名: Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides
作者: Wei, You Chuan1, 2; Pan, Ting Shuang2, 3; Chang, Ming Xian2; Huang, Bei2; Xu, Zhen2; Luo, Ting Rong1; Nie, P.2
通讯作者: Luo, TR, Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, 100 Daxue Rd, Nanning 530004, Guangxi Autonom, Peoples R China ; tingrongluo@gxu.edu.cn ; pinnie@ihb.ac.cn
关键词: Toll-like receptor ; TLR 1 ; TLR2 ; Expression ; Orange-spotted grouper
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2011-06-15
DOI: 10.1016/j.vetimm.2011.02.016
卷: 141, 期:3-4, 页:173-182
收录类别: SCI
文章类型: Article
部门归属: [Wei, You Chuan; Luo, Ting Rong] Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Nanning 530004, Guangxi Autonom, Peoples R China; [Wei, You Chuan; Pan, Ting Shuang; Chang, Ming Xian; Huang, Bei; Xu, Zhen; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Pan, Ting Shuang] Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Anhui, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: The two Toll-like receptors, TLR1 and TLR2 were cloned from orange-spotted grouper, Epinephelus coioides, an important teleost fish in mariculture of Asia. The cDNA sequences of TLR1 and TLR2 are 3195 and 3439 bp long, with an open reading frame (ORF) of 2406 and 2466 bp, encoding 801 and 821 amino acids, respectively. The TLR family motifs, i.e. leucine-rich repeat (LRR) domains and Toll/interleukin (IL)-1 receptor (TIR) domains are conserved in the TLR1 and TLR2, with nine and ten leucine-rich repeat (LRR) domains, and with one TIR domain, respectively. The TLR1 and TLR2 had a constitutive expression in examined organs/tissues of nave orange-spotted grouper, and an increased expression of TLR1 and TLR2 at mRNA level was observed in immune organs such as in spleen of LPS and Poly(I:C) stimulated fish. An increased expression of TLR1 and TLR2 was also recorded in immune organs of the fish injected with the bacterial pathogen, Vibrio alginolyticus. Similarly, a significant rise in the expression of MyD88, an adaptor molecule which forms signalling complex with intracellular TIR domain, thus leading to the production of pro-inflammatory cytokines, such as IL-1 beta, was also observed in the LPS- and Poly(I:C)-stimulated, and V. alginolyticus-infected fish, indicating the possible role of TLR1 and TLR2 in the MyD88 signalling pathway. However, the mechanism involved in the increased expression of TLR1 and TLR2 following LPS and Poly(I:C) stimulation is at present unknown in fish, and further research should be carried out to identify ligands of fish TLR1 and TLR2 in order to understand the function of these receptors. (C) 2011 Elsevier B.V. All rights reserved.
英文摘要: The two Toll-like receptors, TLR1 and TLR2 were cloned from orange-spotted grouper, Epinephelus coioides, an important teleost fish in mariculture of Asia. The cDNA sequences of TLR1 and TLR2 are 3195 and 3439 bp long, with an open reading frame (ORF) of 2406 and 2466 bp, encoding 801 and 821 amino acids, respectively. The TLR family motifs, i.e. leucine-rich repeat (LRR) domains and Toll/interleukin (IL)-1 receptor (TIR) domains are conserved in the TLR1 and TLR2, with nine and ten leucine-rich repeat (LRR) domains, and with one TIR domain, respectively. The TLR1 and TLR2 had a constitutive expression in examined organs/tissues of nave orange-spotted grouper, and an increased expression of TLR1 and TLR2 at mRNA level was observed in immune organs such as in spleen of LPS and Poly(I:C) stimulated fish. An increased expression of TLR1 and TLR2 was also recorded in immune organs of the fish injected with the bacterial pathogen, Vibrio alginolyticus. Similarly, a significant rise in the expression of MyD88, an adaptor molecule which forms signalling complex with intracellular TIR domain, thus leading to the production of pro-inflammatory cytokines, such as IL-1 beta, was also observed in the LPS- and Poly(I:C)-stimulated, and V. alginolyticus-infected fish, indicating the possible role of TLR1 and TLR2 in the MyD88 signalling pathway. However, the mechanism involved in the increased expression of TLR1 and TLR2 following LPS and Poly(I:C) stimulation is at present unknown in fish, and further research should be carried out to identify ligands of fish TLR1 and TLR2 in order to understand the function of these receptors. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: PARALICHTHYS-OLIVACEUS ; JAPANESE FLOUNDER ; INNATE IMMUNITY ; GENE FAMILY ; RECOGNITION ; EVOLUTION ; ZEBRAFISH ; MYD88 ; PUFFERFISH ; ADAPTERS
语种: 英语
WOS记录号: WOS:000291139200001
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15636
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Nanning 530004, Guangxi Autonom, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
3.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Anhui, Peoples R China

Recommended Citation:
Wei, You Chuan; Pan, Ting Shuang; Chang, Ming Xian; Huang, Bei; Xu, Zhen; Luo, Ting Rong; Nie, P..Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2011,141(40606):173-182
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