中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis
作者: Guo, H. Z. ; Zou, P. F. ; Fu, J. P. ; Guo, Z. ; Zhu, B. K. ; Nie, P. ; Chang, M. X.
通讯作者: Chang, MX, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; mingxianchang@ihb.ac.cn
关键词: Eriocheir sinensis ; cDNA library ; C-type lectin-like domain (CTLD)-containing proteins ; Expression
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2011
卷: 30, 期:2, 页:515-524
收录类别: SCI
部门归属: [Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Nie, P.; Chang, M. X.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Nie, P.; Chang, M. X.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Guo, H. Z.; Zhu, B. K.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
资助者: National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083]
摘要: Chinese mitten crab Eriocheir sinensis is one of the most important aquaculture crustacean species in China. A cDNA library was constructed from mixed tissues of E. sinensis challenged with LPS. Eight genes involved in immune response were identified from 319 single colonies. Among them, two different C-type lectin-like domain (CTLD)-containing proteins were firstly identified in Chinese mitten crab. The full-length cDNA sequences of two C-type lectin-like domain (CTLD)-containing proteins named E5CTLDcp-1 and EsCTLDcp-2 were cloned by 5' RACE. The deduced amino acid sequences of E5CTLDcp-1 and E5CTLDcp-2 possessed several conserved features of C-type lectin subfamily. The tissue distribution of EsCTLDcp-1 and EsCTLDcp-2 was examined by Real-time PCR. In the normal Chinese mitten crab, the expression of E5CTLDcp-2 was detected in all tested tissues such as haemolymph, muscle, intestine, gill, heart, gonad and hepatopancreas, whereas in muscle, intestine, gill, heart and hepatopancreas for EsCTLDcp-1. The highest expressions of EsCTLDcp-1 and E5CliDcp-2 were both observed in hepatopancreas. LPS significantly induced the expression of EsCTLDcp-1 and EsCTLDcp-2 in the hepatopancreas at the different time points. The induced fold change of E5CTLDcp-1 and EsCTLDcp-2 increased significantly from 2 h for EsCTLDcp-1 and 4 h for E5CTLDcp-2, and reached a maximum at 12 h, then dropped at 24 h. A differential pattern was found in Chinese mitten crab challenged with Chinese mitten crab pathogen Aeromonas hydrophila. The expression of EsCTLDcp-1 increased significantly at 2 h post-challenge crabs with A. hydrophila, then decreased at 4 h and 8 h, after that increased at 12 h and 24 h. The expression of EsCTLDcp-2 was decreased at the all time points. All these data suggest a differential role of E5CILDcp-1 and EsCTLDcp-2 in the crab innate immune response to bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000288305300010
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15632
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis.pdf(901KB)----开放获取--View Download

Recommended Citation:
Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Zhu, B. K.; Nie, P.; Chang, M. X..Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis,FISH & SHELLFISH IMMUNOLOGY,2011,30(2):515-524
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Guo, H. Z.]'s Articles
[Zou, P. F.]'s Articles
[Fu, J. P.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Guo, H. Z.]‘s Articles
[Zou, P. F.]‘s Articles
[Fu, J. P.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace