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Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis
Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Zhu, B. K.; Nie, P.; Chang, M. X.; Chang, MX, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; mingxianchang@ihb.ac.cn
2011
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume30Issue:2Pages:515-524
AbstractChinese mitten crab Eriocheir sinensis is one of the most important aquaculture crustacean species in China. A cDNA library was constructed from mixed tissues of E. sinensis challenged with LPS. Eight genes involved in immune response were identified from 319 single colonies. Among them, two different C-type lectin-like domain (CTLD)-containing proteins were firstly identified in Chinese mitten crab. The full-length cDNA sequences of two C-type lectin-like domain (CTLD)-containing proteins named E5CTLDcp-1 and EsCTLDcp-2 were cloned by 5' RACE. The deduced amino acid sequences of E5CTLDcp-1 and E5CTLDcp-2 possessed several conserved features of C-type lectin subfamily. The tissue distribution of EsCTLDcp-1 and EsCTLDcp-2 was examined by Real-time PCR. In the normal Chinese mitten crab, the expression of E5CTLDcp-2 was detected in all tested tissues such as haemolymph, muscle, intestine, gill, heart, gonad and hepatopancreas, whereas in muscle, intestine, gill, heart and hepatopancreas for EsCTLDcp-1. The highest expressions of EsCTLDcp-1 and E5CliDcp-2 were both observed in hepatopancreas. LPS significantly induced the expression of EsCTLDcp-1 and EsCTLDcp-2 in the hepatopancreas at the different time points. The induced fold change of E5CTLDcp-1 and EsCTLDcp-2 increased significantly from 2 h for EsCTLDcp-1 and 4 h for E5CTLDcp-2, and reached a maximum at 12 h, then dropped at 24 h. A differential pattern was found in Chinese mitten crab challenged with Chinese mitten crab pathogen Aeromonas hydrophila. The expression of EsCTLDcp-1 increased significantly at 2 h post-challenge crabs with A. hydrophila, then decreased at 4 h and 8 h, after that increased at 12 h and 24 h. The expression of EsCTLDcp-2 was decreased at the all time points. All these data suggest a differential role of E5CILDcp-1 and EsCTLDcp-2 in the crab innate immune response to bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordEriocheir Sinensis Cdna Library C-type Lectin-like Domain (Ctld)-containing Proteins Expression
Department[Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Nie, P.; Chang, M. X.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Guo, H. Z.; Zou, P. F.; Fu, J. P.; Guo, Z.; Nie, P.; Chang, M. X.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Guo, H. Z.; Zhu, B. K.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083]
Indexed BySCI
Language英语
WOS IDWOS:000288305300010
Funding OrganizationNational Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] ; National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15632
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChang, MX, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; mingxianchang@ihb.ac.cn
Recommended Citation
GB/T 7714
Guo, H. Z.,Zou, P. F.,Fu, J. P.,et al. Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(2):515-524.
APA Guo, H. Z..,Zou, P. F..,Fu, J. P..,Guo, Z..,Zhu, B. K..,...&mingxianchang@ihb.ac.cn.(2011).Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,30(2),515-524.
MLA Guo, H. Z.,et al."Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 30.2(2011):515-524.
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