IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
Guan, Bo; Ma, Hong; Wang, Yaping; Hu, Yuanlei; Lin, Zhongping; Zhu, Zuoyan; Hu, Wei; Hu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; huyl@pku.edu.cn; huwei@ihb.ac.cn
2011
Source PublicationMARINE BIOTECHNOLOGY
Volume13Issue:2Pages:336-344
AbstractAquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp beta-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F-2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O-2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish.
KeywordVitreoscilla Hemoglobin (Vhb) Hypoxia Zebrafish (Danio Rerio) Transgenic Fish
Department[Guan, Bo; Wang, Yaping; Zhu, Zuoyan; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guan, Bo] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Ma, Hong; Hu, Yuanlei; Lin, Zhongping] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
Funding Organization863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804]
Indexed BySCI
Funding Organization863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804] ; 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15628
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; huyl@pku.edu.cn; huwei@ihb.ac.cn
Recommended Citation
GB/T 7714
Guan, Bo,Ma, Hong,Wang, Yaping,et al. Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)[J]. MARINE BIOTECHNOLOGY,2011,13(2):336-344.
APA Guan, Bo.,Ma, Hong.,Wang, Yaping.,Hu, Yuanlei.,Lin, Zhongping.,...&huwei@ihb.ac.cn.(2011).Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).MARINE BIOTECHNOLOGY,13(2),336-344.
MLA Guan, Bo,et al."Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)".MARINE BIOTECHNOLOGY 13.2(2011):336-344.
Files in This Item:
File Name/Size DocType Version Access License
Vitreoscilla Hemoglo(223KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Guan, Bo]'s Articles
[Ma, Hong]'s Articles
[Wang, Yaping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Guan, Bo]'s Articles
[Ma, Hong]'s Articles
[Wang, Yaping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Guan, Bo]'s Articles
[Ma, Hong]'s Articles
[Wang, Yaping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.