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学科主题: Biotechnology & Applied Microbiology; Marine & Freshwater Biology
题名: Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
作者: Guan, Bo ; Ma, Hong ; Wang, Yaping ; Hu, Yuanlei ; Lin, Zhongping ; Zhu, Zuoyan ; Hu, Wei
通讯作者: Hu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; huyl@pku.edu.cn ; huwei@ihb.ac.cn
关键词: Vitreoscilla hemoglobin (VHb) ; Hypoxia ; Zebrafish (Danio rerio) ; Transgenic fish
刊名: MARINE BIOTECHNOLOGY
发表日期: 2011
卷: 13, 期:2, 页:336-344
收录类别: SCI
部门归属: [Guan, Bo; Wang, Yaping; Zhu, Zuoyan; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guan, Bo] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Ma, Hong; Hu, Yuanlei; Lin, Zhongping] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
资助者: 863 High-Technology Project [2007AA10Z186]; National Natural Science Foundation of China [30623001]; Development Plan of the State Key Fundamental Research of China [2009CB118804]
摘要: Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp beta-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F-2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O-2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish.
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15628
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Recommended Citation:
Guan, Bo; Ma, Hong; Wang, Yaping; Hu, Yuanlei; Lin, Zhongping; Zhu, Zuoyan; Hu, Wei.Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio) (SIC收录),MARINE BIOTECHNOLOGY,2011,13(2):336-344
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