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学科主题: Fisheries; Marine & Freshwater Biology
题名: Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages
作者: Cao, H. ; Zhou, R. J. ; Wei, Q. W. ; Li, C. J. ; Gui, J. F.
通讯作者: Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; jfgui@ihb.ac.cn
关键词: CLONING ; PROTEIN ; PITUITARIES ; PREGNANCY ; PLACENTA ; CDNA ; FISH
刊名: JOURNAL OF APPLIED ICHTHYOLOGY
发表日期: 2011
卷: 27, 期:2, 页:501-504
收录类别: SCI
部门归属: [Cao, H.; Gui, J. F.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Cao, H.; Zhou, R. J.] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China; [Wei, Q. W.; Li, C. J.] Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Yangtze River Fisheries Res Inst, Jinzhou, Peoples R China
资助者: National Natural Science Foundation of China [30900139]
摘要: Chinese sturgeon (Acipenser sinensis Gray) has a fast growth rate starting in early larval stage. Likely, the fast growth is attributed to the growth hormone (GH); an essential polypeptide required for growth and development of vertebrates. In this study, GH cDNA was cloned from a pituitary library. The AsGH cDNA consists approximately 954 bp in size including a 16 bp 5'-untranslated region and 296 bp 3'-untranslated region. The open reading frame (642 bp) encodes a 214 amino acids (aa), which represents the precursor composed of a 25 aa signal peptide followed by a 189 aa mature polypeptide. The polyclonal antibody was prepared from the in vitro expressed mature protein. Western blot analysis showed a constant GH expression of a monomeric form in the 13 embryo stages and in several larval stages. Interestingly, two dimeric bands of GH were detected since day 3 in the hatched larvae. Our findings suggest that the GH may play important physiological functions in embryonic and larval development of Chinese sturgeon.
语种: 英语
WOS记录号: WOS:000288864600054
ISSN号: 0175-8659
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15624
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Recommended Citation:
Cao, H.; Zhou, R. J.; Wei, Q. W.; Li, C. J.; Gui, J. F..Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages,JOURNAL OF APPLIED ICHTHYOLOGY,2011,27(2):501-504
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