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Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages
Cao, H.; Zhou, R. J.; Wei, Q. W.; Li, C. J.; Gui, J. F.; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; jfgui@ihb.ac.cn
2011
Source PublicationJOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
Volume27Issue:2Pages:501-504
AbstractChinese sturgeon (Acipenser sinensis Gray) has a fast growth rate starting in early larval stage. Likely, the fast growth is attributed to the growth hormone (GH); an essential polypeptide required for growth and development of vertebrates. In this study, GH cDNA was cloned from a pituitary library. The AsGH cDNA consists approximately 954 bp in size including a 16 bp 5'-untranslated region and 296 bp 3'-untranslated region. The open reading frame (642 bp) encodes a 214 amino acids (aa), which represents the precursor composed of a 25 aa signal peptide followed by a 189 aa mature polypeptide. The polyclonal antibody was prepared from the in vitro expressed mature protein. Western blot analysis showed a constant GH expression of a monomeric form in the 13 embryo stages and in several larval stages. Interestingly, two dimeric bands of GH were detected since day 3 in the hatched larvae. Our findings suggest that the GH may play important physiological functions in embryonic and larval development of Chinese sturgeon.
KeywordCloning Protein Pituitaries Pregnancy Placenta Cdna Fish
Department[Cao, H.; Gui, J. F.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Cao, H.; Zhou, R. J.] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China; [Wei, Q. W.; Li, C. J.] Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Yangtze River Fisheries Res Inst, Jinzhou, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
Funding OrganizationNational Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139]
Indexed BySCI
Language英语
WOS IDWOS:000288864600054
Funding OrganizationNational Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139] ; National Natural Science Foundation of China [30900139]
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15624
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; jfgui@ihb.ac.cn
Recommended Citation
GB/T 7714
Cao, H.,Zhou, R. J.,Wei, Q. W.,et al. Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2011,27(2):501-504.
APA Cao, H..,Zhou, R. J..,Wei, Q. W..,Li, C. J..,Gui, J. F..,...&jfgui@ihb.ac.cn.(2011).Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages.JOURNAL OF APPLIED ICHTHYOLOGY,27(2),501-504.
MLA Cao, H.,et al."Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages".JOURNAL OF APPLIED ICHTHYOLOGY 27.2(2011):501-504.
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