中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
学科主题: Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
题名: Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct
作者: Ahmed, A. S. Ishtiaq ; Xiong, Feng ; Pang, Shao-Chen ; He, Mu-Dan ; Waters, Michael J. ; Zhu, Zuo-Yan ; Sun, Yong-Hua
通讯作者: Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; sunyonghua@gmail.com
关键词: GH ; GHR ; Constitutively active GHR ; Protein design ; Zebrafish
刊名: TRANSGENIC RESEARCH
发表日期: 2011
卷: 20, 期:3, 页:557-567
收录类别: SCI
部门归属: [Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan; Zhu, Zuo-Yan; Sun, Yong-Hua] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Waters, Michael J.] Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia; [Waters, Michael J.] Univ Queensland, Ctr Mol & Cellular Biol, St Lucia, Qld 4072, Australia; [Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
资助者: China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS
摘要: Growth hormone (GH) gene transfer can markedly increase growth in transgenic fish. In the present study we have developed a transcriptional assay to evaluate GH-signal activation (GHSA) in zebrafish embryos. By analyzing the transcription of c-fos and igf1, and the promoter activity of spi2.1, in zebrafish embryos injected with different constructs, we found that overexpression of either GH or growth hormone receptor (GHR) resulted in GHSA, while a synergetic overexpression of GH and GHR gave greater activation. Conversely, overexpression of a C-terminal truncated dominant-negative GHR (Delta C-GHR) efficiently blocked GHSA epistatic to GH overexpression, demonstrating the requirement for a full GHR homodimer in signaling. In view of the importance of signal-competent GHR dimerization by extracellular GH, we introduced into zebrafish embryos a constitutively activated GHR (CA-GHR) construct, which protein products constitutively dimerize the GHR productively by Jun-zippers to activate downstream signaling in vitro. Importantly, overexpression of CA-GHR led to markedly higher level of GHSA than the synergetic overexpression of GH and GHR. CA-GHR transgenic zebrafish were then studied in a growth trial. The transgenic zebrafish showed higher growth rate than the control fish, which was not achievable by GH transgenesis in these zebrafish. Our study demonstrates GH-independent growth by CA-GHR in vivo which bypasses normal IGF-1 feedback control of GH secretion. This provides a novel means of producing growth enhanced transgenic animals based on molecular protein design.
语种: 英语
WOS记录号: WOS:000290446200011
ISSN号: 0962-8819
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15620
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct.pdf(508KB)----开放获取--View Download

Recommended Citation:
Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan; Waters, Michael J.; Zhu, Zuo-Yan; Sun, Yong-Hua.Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct,TRANSGENIC RESEARCH,2011,20(3):557-567
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ahmed, A. S. Ishtiaq]'s Articles
[Xiong, Feng]'s Articles
[Pang, Shao-Chen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ahmed, A. S. Ishtiaq]‘s Articles
[Xiong, Feng]‘s Articles
[Pang, Shao-Chen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace