IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct
Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan; Waters, Michael J.; Zhu, Zuo-Yan; Sun, Yong-Hua; Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; sunyonghua@gmail.com
2011
Source PublicationTRANSGENIC RESEARCH
ISSN0962-8819
Volume20Issue:3Pages:557-567
AbstractGrowth hormone (GH) gene transfer can markedly increase growth in transgenic fish. In the present study we have developed a transcriptional assay to evaluate GH-signal activation (GHSA) in zebrafish embryos. By analyzing the transcription of c-fos and igf1, and the promoter activity of spi2.1, in zebrafish embryos injected with different constructs, we found that overexpression of either GH or growth hormone receptor (GHR) resulted in GHSA, while a synergetic overexpression of GH and GHR gave greater activation. Conversely, overexpression of a C-terminal truncated dominant-negative GHR (Delta C-GHR) efficiently blocked GHSA epistatic to GH overexpression, demonstrating the requirement for a full GHR homodimer in signaling. In view of the importance of signal-competent GHR dimerization by extracellular GH, we introduced into zebrafish embryos a constitutively activated GHR (CA-GHR) construct, which protein products constitutively dimerize the GHR productively by Jun-zippers to activate downstream signaling in vitro. Importantly, overexpression of CA-GHR led to markedly higher level of GHSA than the synergetic overexpression of GH and GHR. CA-GHR transgenic zebrafish were then studied in a growth trial. The transgenic zebrafish showed higher growth rate than the control fish, which was not achievable by GH transgenesis in these zebrafish. Our study demonstrates GH-independent growth by CA-GHR in vivo which bypasses normal IGF-1 feedback control of GH secretion. This provides a novel means of producing growth enhanced transgenic animals based on molecular protein design.
KeywordGh Ghr Constitutively Active Ghr Protein Design Zebrafish
Department[Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan; Zhu, Zuo-Yan; Sun, Yong-Hua] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Waters, Michael J.] Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia; [Waters, Michael J.] Univ Queensland, Ctr Mol & Cellular Biol, St Lucia, Qld 4072, Australia; [Ahmed, A. S. Ishtiaq; Xiong, Feng; Pang, Shao-Chen; He, Mu-Dan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationChina 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS
Indexed BySCI
Language英语
WOS IDWOS:000290446200011
Funding OrganizationChina 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS ; China 973 Project [2010CB126306]; National Natural Science Foundation of China [30972248, 30771100]; China 863 project [2007AA10Z186]; Youth Sunshine Project of Wuhan City ; Institute of Hydrobiology, CAS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15620
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; sunyonghua@gmail.com
Recommended Citation
GB/T 7714
Ahmed, A. S. Ishtiaq,Xiong, Feng,Pang, Shao-Chen,et al. Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct[J]. TRANSGENIC RESEARCH,2011,20(3):557-567.
APA Ahmed, A. S. Ishtiaq.,Xiong, Feng.,Pang, Shao-Chen.,He, Mu-Dan.,Waters, Michael J..,...&sunyonghua@gmail.com.(2011).Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct.TRANSGENIC RESEARCH,20(3),557-567.
MLA Ahmed, A. S. Ishtiaq,et al."Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct".TRANSGENIC RESEARCH 20.3(2011):557-567.
Files in This Item:
File Name/Size DocType Version Access License
Activation of GH sig(508KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ahmed, A. S. Ishtiaq]'s Articles
[Xiong, Feng]'s Articles
[Pang, Shao-Chen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ahmed, A. S. Ishtiaq]'s Articles
[Xiong, Feng]'s Articles
[Pang, Shao-Chen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ahmed, A. S. Ishtiaq]'s Articles
[Xiong, Feng]'s Articles
[Pang, Shao-Chen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.