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学科主题: Biochemical Research Methods; Biochemistry & Molecular Biology
题名: Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR
作者: Li, Guangyu ; Chen, Jun ; Xie, Ping ; Jiang, Yan ; Wu, Laiyan ; Zhang, Xuezhen
通讯作者: Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China ; xieping@ihb.ac.cn
关键词: Animal proteomics ; Developmental toxicity ; Gene expression ; Microcystin-leucine-arginine ; Zebrafish
刊名: PROTEOMICS
发表日期: 2011
卷: 11, 期:10, 页:2003-2018
收录类别: SCI
部门归属: [Li, Guangyu; Chen, Jun; Xie, Ping; Jiang, Yan; Wu, Laiyan] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
资助者: National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]
摘要: Microcystin-leucine-arginine (MCLR) is the most toxic and the most commonly encountered variant of microcystins (MCs) in aquatic environment, and it has the potential for developmental toxicity. A number of previous studies have described the developing toxicity of MCLR based on conventional toxicological indices. However, the molecular mechanisms by which it expresses its toxicity during the early development remain largely unknown. To further our understanding of mechanisms of action and identify the potential protein biomarkers for MCLR exposure, a proteomic analysis was performed on developing zebrafish embryos exposed to 0.5 mg/L MCLR until 96 hours post-fertilization. 2-DE combined with MS was employed to detect and identify the protein profiles. Results showed that 75 spots from the 0.5 mg/L MCLR condition showed a significant increase or decrease in abundance compared with the control. In total, 40 proteins were identified. These proteins were mainly included in process related to oxidative stress, energetic metabolism, and the cytoskeleton assembly. MCLR exposure also affects the expression of the subunits of protein phosphatases 2A. Furthermore, the proteomic and transcriptional analysis of nine proteins was determined by Western blot and quantitative real-time PCR due to their correlation with the known MCLR toxic mechanisms. The consistent and discrepant results between protein and mRNA levels indicated complicated regulatory mechanisms of gene expression in response to MCLR exposure.
语种: 英语
WOS记录号: WOS:000290486200012
ISSN号: 1615-9853
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15578
Appears in Collections:淡水生态学研究中心_期刊论文

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Recommended Citation:
Li, Guangyu; Chen, Jun; Xie, Ping; Jiang, Yan; Wu, Laiyan; Zhang, Xuezhen.Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR,PROTEOMICS,2011,11(10):2003-2018
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文件名: Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR.pdf
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