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题名: Isolation and characterization of microsatellite loci in Rhinogobio ventralis
作者: Xu, Nian1, 2, 3, 4; Shi, Fang1, 2, 3, 4; Xiong, Meihua1, 2; Que, Yanfu1, 2; Yang, Zhong1, 2; Zhu, Bin1, 2; Chang, Jianbo1, 2
通讯作者: Chang, JB, Minist Water Resources, Inst Hydroecol, 578th Xiongchu Ave, Wuhan 430079, Hubei, Peoples R China ; jbchang@mail.ihe.ac.cn
关键词: Rhinogobio ventralis ; Microsatellites ; Polymorphic loci ; Population genetics
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2010-09-01
DOI: 10.1007/s12686-009-9024-9
卷: 2, 页:1-3
收录类别: SCI
文章类型: Article
部门归属: [Xu, Nian; Shi, Fang; Xiong, Meihua; Que, Yanfu; Yang, Zhong; Zhu, Bin; Chang, Jianbo] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Hubei, Peoples R China; [Xu, Nian; Shi, Fang; Xiong, Meihua; Que, Yanfu; Yang, Zhong; Zhu, Bin; Chang, Jianbo] Chinese Acad Sci, Wuhan 430079, Hubei, Peoples R China; [Xu, Nian; Shi, Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Xu, Nian; Shi, Fang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: hydro-biodiversity monitoring at Pengshui hydropower station on Wujiang River [PS/HB011-2006]
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
摘要: From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.
英文摘要: From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.
关键词[WOS]: DNA
语种: 英语
WOS记录号: WOS:000290413000001
ISSN号: 1877-7252
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15576
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan 430079, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Xu, Nian; Shi, Fang; Xiong, Meihua; Que, Yanfu; Yang, Zhong; Zhu, Bin; Chang, Jianbo.Isolation and characterization of microsatellite loci in Rhinogobio ventralis,CONSERVATION GENETICS RESOURCES,2010,2():1-3
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