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Cloning the interferon regulatory factor 1 gene in lungfish (Protopterus annectens) and its molecular evolution among sarcopterygians
Gan XiaoNi; Wang DengQiang; Wang XuZhen; Chen XinWen; Chen, XW, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; chenxw@wh.iov.cn
2011
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume56Issue:17Pages:1782-1786
AbstractSarcopterygians is an important vertebrate clade that includes crossopterygians and tetrapods. Crossopterygians are lobe-finned fish that include lungfish and coelacanths. Tetrapods include amphibians, reptiles, avians and mammals. To compare the interferon regulatory factor 1 (irf-1) gene structure and to explore phylogenetic relationships among sarcopterygians, we cloned the cDNA sequence of irf-1 from lungfish and compared it with irf-1 orthologs in other sarcopterygian species. The lungfish is a primitive sarcopterygian that occupies a very important position in vertebrate phylogeny. Interferon regulatory factors (IRFs) are a family of proteins involved in innate immunity. To date, 11 IRF family members have been reported. All IRFs share homology in the first 115 amino acids, which encompasses a DNA binding domain containing a characteristic repeat of 5 tryptophan residues separated by 10-18 amino acids. IRF-1 and IRF-2 were the first members of this family to be reported and they have a very important role in innate immunity. However, studies of the irf-1 and irf-2 genes are mostly confined to mammals; very few non-mammalian irf-1 genes have been reported. Consistent with the irf-1 gene sequences already published, the first 345 nucleotides of lungfish irf-1 are highly conserved. At the carboxyl terminal a C-terminal transactivating region motif and an interferon associated domain (IAD2) were identified. 417 million years separate the present from the closest common ancestor of lungfish and tetrapods; however, the irf-1 genes among sarcopterygians are highly conserved and have very obvious phylogenetic relationships. Also the interrelationship tree of sarcopterygians, based on IRF-1 amino acid sequences, is identical with trees produced using other data, such as morphological characteristics or mitochondrial gene sequences.
KeywordIrf-1 Innate Immunity Sarcopterygii Molecular Evolution Phylogeny
Department[Gan XiaoNi; Chen XinWen] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; [Gan XiaoNi; Wang DengQiang; Wang XuZhen] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Gan XiaoNi] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Subject AreaMultidisciplinary Sciences
Funding OrganizationNational Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500]
Indexed BySCI
Language英语
WOS IDWOS:000291259300005
Funding OrganizationNational Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500] ; National Key Basic Research Program of China [HCV2009CB522500]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15574
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorChen, XW, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; chenxw@wh.iov.cn
Recommended Citation
GB/T 7714
Gan XiaoNi,Wang DengQiang,Wang XuZhen,et al. Cloning the interferon regulatory factor 1 gene in lungfish (Protopterus annectens) and its molecular evolution among sarcopterygians[J]. CHINESE SCIENCE BULLETIN,2011,56(17):1782-1786.
APA Gan XiaoNi,Wang DengQiang,Wang XuZhen,Chen XinWen,Chen, XW, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China,&chenxw@wh.iov.cn.(2011).Cloning the interferon regulatory factor 1 gene in lungfish (Protopterus annectens) and its molecular evolution among sarcopterygians.CHINESE SCIENCE BULLETIN,56(17),1782-1786.
MLA Gan XiaoNi,et al."Cloning the interferon regulatory factor 1 gene in lungfish (Protopterus annectens) and its molecular evolution among sarcopterygians".CHINESE SCIENCE BULLETIN 56.17(2011):1782-1786.
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