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Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae
Chen, Wei; Sommerfeld, Milton; Hu, Qiang; Hu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA; huqiang@asu.edu
2011
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume102Issue:1Pages:135-141
AbstractIn vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd.
KeywordBiofuel Microwave-assisted Staining Green Algae Neutral Lipids Nile Red
Department[Chen, Wei; Sommerfeld, Milton; Hu, Qiang] Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA; [Chen, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Subject AreaAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
Indexed BySCI
Language英语
WOS IDWOS:000285658300020
Citation statistics
Cited Times:97[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15542
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA; huqiang@asu.edu
Recommended Citation
GB/T 7714
Chen, Wei,Sommerfeld, Milton,Hu, Qiang,et al. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae[J]. BIORESOURCE TECHNOLOGY,2011,102(1):135-141.
APA Chen, Wei,Sommerfeld, Milton,Hu, Qiang,Hu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA,&huqiang@asu.edu.(2011).Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae.BIORESOURCE TECHNOLOGY,102(1),135-141.
MLA Chen, Wei,et al."Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae".BIORESOURCE TECHNOLOGY 102.1(2011):135-141.
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