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学科主题: Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
题名: Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae
作者: Chen, Wei ; Sommerfeld, Milton ; Hu, Qiang
通讯作者: Hu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA ; huqiang@asu.edu
关键词: Biofuel ; Microwave-assisted staining ; Green algae ; Neutral lipids ; Nile red
刊名: BIORESOURCE TECHNOLOGY
发表日期: 2011
卷: 102, 期:1, 页:135-141
收录类别: SCI
部门归属: [Chen, Wei; Sommerfeld, Milton; Hu, Qiang] Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA; [Chen, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
摘要: In vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd.
语种: 英语
WOS记录号: WOS:000285658300020
ISSN号: 0960-8524
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15542
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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Recommended Citation:
Chen, Wei; Sommerfeld, Milton; Hu, Qiang.Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae,BIORESOURCE TECHNOLOGY,2011,102(1):135-141
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