IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species
Rao, Yu; Zhong, Liqiao; Liao, Tao; Jin, Shiwei; Wang, Yuzhen; Song, Bo; Li, Jia; Zhang, Xiaohua; Hemmingsen, Sean M.; Xu, Ying; Dai, Heping; Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 So E Lake Rd, Wuhan 430072, Peoples R China; hpdai@ihb.ac.cn
2010
Source PublicationDISEASES OF AQUATIC ORGANISMS
ISSN0177-5103
Volume93Issue:1Pages:83-91
AbstractVarious polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
KeywordVitellogenin Detection Cyprinid Fish Phage Display Scfv Antibody Bioassay Binding Affinity
Department[Rao, Yu; Zhong, Liqiao; Liao, Tao; Jin, Shiwei; Wang, Yuzhen; Song, Bo; Li, Jia; Zhang, Xiaohua; Xu, Ying; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Rao, Yu; Zhong, Liqiao; Li, Jia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Hemmingsen, Sean M.] Natl Res Council Canada, Plant Biotechnol Inst, Saskatoon, SK S7N 0W9, Canada
Subject AreaFisheries ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424]
Indexed BySCI
Language英语
WOS IDWOS:000285305100008
Funding OrganizationNational Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] ; National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424]
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15530
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorDai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 So E Lake Rd, Wuhan 430072, Peoples R China; hpdai@ihb.ac.cn
Recommended Citation
GB/T 7714
Rao, Yu,Zhong, Liqiao,Liao, Tao,et al. Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species[J]. DISEASES OF AQUATIC ORGANISMS,2010,93(1):83-91.
APA Rao, Yu.,Zhong, Liqiao.,Liao, Tao.,Jin, Shiwei.,Wang, Yuzhen.,...&hpdai@ihb.ac.cn.(2010).Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.DISEASES OF AQUATIC ORGANISMS,93(1),83-91.
MLA Rao, Yu,et al."Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species".DISEASES OF AQUATIC ORGANISMS 93.1(2010):83-91.
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