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学科主题: Fisheries; Veterinary Sciences
题名: Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species
作者: Rao, Yu1, 2; Zhong, Liqiao1, 2; Liao, Tao1; Jin, Shiwei1; Wang, Yuzhen1; Song, Bo1; Li, Jia1, 2; Zhang, Xiaohua1; Hemmingsen, Sean M.3; Xu, Ying1; Dai, Heping1
通讯作者: Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 So E Lake Rd, Wuhan 430072, Peoples R China ; hpdai@ihb.ac.cn
关键词: Vitellogenin detection ; Cyprinid fish ; Phage display ; ScFv antibody ; Bioassay ; Binding affinity
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2010-12-07
DOI: 10.3354/dao02268
卷: 93, 期:1, 页:83-91
收录类别: SCI
文章类型: Article
部门归属: [Rao, Yu; Zhong, Liqiao; Liao, Tao; Jin, Shiwei; Wang, Yuzhen; Song, Bo; Li, Jia; Zhang, Xiaohua; Xu, Ying; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Rao, Yu; Zhong, Liqiao; Li, Jia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Hemmingsen, Sean M.] Natl Res Council Canada, Plant Biotechnol Inst, Saskatoon, SK S7N 0W9, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424]
类目[WOS]: Fisheries ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Veterinary Sciences
摘要: Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
英文摘要: Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
关键词[WOS]: ZEBRAFISH DANIO-RERIO ; MINNOW PIMEPHALES-PROMELAS ; LINKED-IMMUNOSORBENT-ASSAY ; ESTROGENIC CHEMICALS ; GOBIOCYPRIS-RARUS ; CARP ; BIOMARKER ; IMMUNOASSAY ; ELISA ; PURIFICATION
语种: 英语
WOS记录号: WOS:000285305100008
ISSN号: 0177-5103
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15530
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Natl Res Council Canada, Plant Biotechnol Inst, Saskatoon, SK S7N 0W9, Canada

Recommended Citation:
Rao, Yu; Zhong, Liqiao; Liao, Tao; Jin, Shiwei; Wang, Yuzhen; Song, Bo; Li, Jia; Zhang, Xiaohua; Hemmingsen, Sean M.; Xu, Ying; Dai, Heping.Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species,DISEASES OF AQUATIC ORGANISMS,2010,93(1):83-91
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