IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish
Long, Yong; Li, Qing; Cui, Zongbin; Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China; zbcui@ihb.ac.cn
2011
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume38Issue:3Pages:1703-1711
AbstractABCC1/MRP1 belongs to the ATP-binding cassette superfamily and its elevated expression is closely associated with the multidrug resistance of various tumor cells. In normal tissues, ABCC1 confers resistance to a wide variety of xenobiotics and toxicants, demonstrating its important roles in tissue defense. Here, we report the cloning and functional characterization of abcc1 gene in zebrafish. This gene is localized on zebrafish chromosome 3 and contains a 4,557 bp open-reading frame. The deduced polypeptide is composed of 1,518 amino acids, which shares 70% identity with human ABCC1. Phylogenetic analysis revealed that ABCC1 proteins from thirteen vertebrate species are highly conserved during evolution. Transcriptional expression of zebrafish abcc1 gene in developing embryos was examined by whole-mount in situ hybridization and real-time PCR. Transcripts of zebrafish abcc1 gene were detectable in four-cell stage embryos, indicating that this gene is maternally expressed. ABCC1 mRNAs were ubiquitously distributed in embryos before 12 h post-fertilization (hpf) and mainly localized in eyes and brain from 24 to 72 hpf, and in gills from 96 to 120 hpf. In addition, zebrafish abcc1 gene was highly expressed in 1-hpf embryos and detected in all adult tissues examined, with highest expression in testis and lowest in heart and liver. Exposure of ZF4 cells and embryos to CdCl2 center dot 2.5H(2)O, HgCl2, Pb(NO3)(2), or Na3AsO4 center dot 12H(2)O significantly induced transcriptional expression of abcc1 gene. Furthermore, overexpression of abcc1 improved the survival rates of embryos exposed to Cd, Hg or As, while overexpression of a abcc1 mutant (ABCC1-G1420D) sensitized zebrafish embryos to toxic metals. These data indicate that zebrafish ABCC1 has crucial roles in heavy metals detoxification.
KeywordAbc Transporter Abcc1 Multidrug Resistance Tissue Defense Heavy Metals
Department[Long, Yong; Li, Qing; Cui, Zongbin] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Long, Yong] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442]
Indexed BySCI
Language英语
WOS IDWOS:000286941400033
Funding OrganizationNational Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442] ; National Natural Science Foundation of China [30671602, 30871442]
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Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15526
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China; zbcui@ihb.ac.cn
Recommended Citation
GB/T 7714
Long, Yong,Li, Qing,Cui, Zongbin,et al. Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish[J]. MOLECULAR BIOLOGY REPORTS,2011,38(3):1703-1711.
APA Long, Yong,Li, Qing,Cui, Zongbin,Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China,&zbcui@ihb.ac.cn.(2011).Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish.MOLECULAR BIOLOGY REPORTS,38(3),1703-1711.
MLA Long, Yong,et al."Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish".MOLECULAR BIOLOGY REPORTS 38.3(2011):1703-1711.
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