IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells
Ge, Feng; Zhang, Liang; Tao, Sheng-Ce; Kitazato, Kaio; Zhang, Zhi-Ping; Zhang, Xian-En; Bi, Li-Jun; Ge, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; gefeng@ihb.ac.cn, blj@sun5.ibp.ac.cn
2011
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume10Issue:2Pages:845-855
AbstractTumor reversion is defined as the process by which cancer cells lose their malignant phenotype. However, relatively little is known about the cellular proteome changes that occur during the reversion process. A biological model of multiple myelonna (MM) reversion was established by using the H-1 parvovirus as a tool to select for revertant cells from MM cells. Isolated revertant cells displayed a strongly suppressed malignant phenotype both in vitro and in vivo. To explore possible mechanisms of MM reversion, the protein profiles of the revertant and parental MM cells were compared using a quantitative proteomic strategy termed SILAC-MS. Our results revealed that 379 proteins were either activated or inhibited during the reversion process, with a much greater proportion of the proteins, including STAT3, TCTP, CDC2, BAG2, and PCNA, being inhibited. Of these, STAT3, which is significantly down regulated, was selected for further functional studies. Inhibition of STAT3 expression by RNA interference resulted in suppression of the malignant phenotype and concomitant down regulation of TCTP expression, suggesting that myeloma reversion operates, at least in part, through inhibition of STAT3. Our results provide novel insights into the mechanisms of tumor reversion and suggest new alternative approaches for MM treatment.
KeywordMultiple Myeloma (Mm) Tumor Reversion Stable Isotope LabelIng By AmIno Acids In Cell Culture (Silac) Signal Transducer And Activator Of Transcription 3 (Stat3)
Department[Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Liang] Singapore Hlth Res Facil, Div Res, Singapore 169611, Singapore; [Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China; [Kitazato, Kaio] Nagasaki Univ, Dept Mol Microbiol & Immunol, Nagasaki 8528521, Japan; [Zhang, Zhi-Ping; Zhang, Xian-En] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China; [Bi, Li-Jun] Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
Subject AreaBiochemical Research Methods
Funding OrganizationChinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS)
Indexed BySCI
Language英语
WOS IDWOS:000286868500044
Funding OrganizationChinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS)
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15522
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; gefeng@ihb.ac.cn, blj@sun5.ibp.ac.cn
Recommended Citation
GB/T 7714
Ge, Feng,Zhang, Liang,Tao, Sheng-Ce,et al. Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(2):845-855.
APA Ge, Feng.,Zhang, Liang.,Tao, Sheng-Ce.,Kitazato, Kaio.,Zhang, Zhi-Ping.,...&gefeng@ihb.ac.cn, blj@sun5.ibp.ac.cn.(2011).Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells.JOURNAL OF PROTEOME RESEARCH,10(2),845-855.
MLA Ge, Feng,et al."Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells".JOURNAL OF PROTEOME RESEARCH 10.2(2011):845-855.
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