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Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths
Li, Xiaoyan; Anken, Ralf H.; Wang, Gaohong; Hilbig, Reinhard; Liu, Yongding; Liu, YD, Chinese Acad Sci, Inst Hydrobiol, Donghunanlu 7, Wuhan 430072, Hunan, Peoples R China; ralf.anken@dlr.de, liuyd@ihb.ac.cn
2011
Source PublicationMICROGRAVITY SCIENCE AND TECHNOLOGY
ISSN0938-0108
Volume23Issue:1Pages:13-18
AbstractStimulus dependence is a general feature of developing sensory systems. It has been shown earlier that the growth of otoliths of late-stage Cichlid fish (Oreochromis mossambicus) and Zebrafish (Danio rerio) was slowed down by hypergravity, whereas microgravity during spaceflight yielded an opposite effect, i.e., larger than 1 g otoliths, in Swordtail (Xiphophorus helleri) late-stage embryos. Using ground-based techniques to apply simulated weightlessness, long-term clinorotation (exposure on a fast-rotating clinostat with one axis of rotation for 7 days) led to larger than 1 g otoliths in late-stage Cichlid fish, which is fully in line with the results obtained on Swordtails from spaceflight. Hitherto, early-staged fish have not yet been subjected to (simulated or real) long-term (i.e., more than 3 or 4 days) weightlessness to investigate otolith growth. The present study was carried out in order to fill this gap. Therefore, we subjected Zebrafish at a somite-stage to Wall Vessel Rotation (WVR; a method regarded to provide simulated weightlessness), when the anlage of the inner ear already is present (10 h post fertilisation, hpf). Siblings were maintained under WVR for 3, 6, 9 and 12 days. Further short-term experiments (3 days) were carried out on 10 hpf animals as well as on very early larvae (1 K cell stage, 3 hpf) at two different rotation speeds. WVR (both rotation speeds) had no effect on otolith biogenesis in both stages as all otoliths were present after the experiments. In comparison with 1 g controls, WVR had significantly increased otolith growth (normalised by fish length) after 3 and 6 days of exposure, but significant differences of otolith growth between experimental animals and controls were not found after 9 and 12 days. In conclusion, WVR (at least within a time-span of exposure of up to 6 days) brings, comparable to the situation in real microgravity, a kind of feedback mechanism into action, resulting in larger otoliths. Later, possible effects of WVR might be overruled by normal allometric growth since the action of the feedback mechanism may be discontinued in the course of an adaptation.
KeywordVestibular System Development Functional Weightlessness
Department[Li, Xiaoyan; Wang, Gaohong; Liu, Yongding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China; [Anken, Ralf H.] German Aerosp Ctr, Inst Aerosp Med, D-51147 Cologne, Germany; [Anken, Ralf H.; Hilbig, Reinhard] Univ Hohenheim, Inst Zool, D-70593 Stuttgart, Germany
Subject AreaEngineering, Aerospace ; Thermodynamics ; Mechanics
Funding OrganizationNational Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093]
Indexed BySCI
Language英语
WOS IDWOS:000286396000002
Funding OrganizationNational Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093] ; National Natural Science foundation of China [30400093]
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15510
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, Inst Hydrobiol, Donghunanlu 7, Wuhan 430072, Hunan, Peoples R China; ralf.anken@dlr.de, liuyd@ihb.ac.cn
Recommended Citation
GB/T 7714
Li, Xiaoyan,Anken, Ralf H.,Wang, Gaohong,et al. Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2011,23(1):13-18.
APA Li, Xiaoyan.,Anken, Ralf H..,Wang, Gaohong.,Hilbig, Reinhard.,Liu, Yongding.,...&ralf.anken@dlr.de, liuyd@ihb.ac.cn.(2011).Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths.MICROGRAVITY SCIENCE AND TECHNOLOGY,23(1),13-18.
MLA Li, Xiaoyan,et al."Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths".MICROGRAVITY SCIENCE AND TECHNOLOGY 23.1(2011):13-18.
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