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学科主题: Engineering ; Environmental; Environmental Sciences
题名: Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis
作者: Hou, Yansong ; Liang, Wei ; Zhang, Liping ; Cheng, Shuiping ; He, Feng ; Wu, Zhenbin
通讯作者: Liang, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; liangwei02@tsinghua.org.cn, wuzb@ihb.ac.cn
关键词: high-performance liquid chromatographic (HPLC) ; algae ; pigment ; chemotaxonomy
刊名: FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA
发表日期: 2011
卷: 5, 期:1, 页:84-91
收录类别: SCI
部门归属: [Hou, Yansong; Liang, Wei; Zhang, Liping; Cheng, Shuiping; He, Feng; Wu, Zhenbin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Hou, Yansong] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
资助者: National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015]
摘要: The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reversephase C-18 nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide a, 19'-butanoyloxyfucoxanthin, chlorophyll c (1) + c (2), phephorbides a, peridinin, methyl-chlorophyllide a, fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b allomer, chlorophyll b, chlorophyll a allomer, chlorophyll a, and beta,beta-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15508
Appears in Collections:水环境工程研究中心_期刊论文

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Hou, Yansong; Liang, Wei; Zhang, Liping; Cheng, Shuiping; He, Feng; Wu, Zhenbin.Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis,FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA,2011,5(1):84-91
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