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Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis
Hou, Yansong; Liang, Wei; Zhang, Liping; Cheng, Shuiping; He, Feng; Wu, Zhenbin; Liang, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; liangwei02@tsinghua.org.cn, wuzb@ihb.ac.cn
2011
Source PublicationFRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA
Volume5Issue:1Pages:84-91
AbstractThe study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reversephase C-18 nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide a, 19'-butanoyloxyfucoxanthin, chlorophyll c (1) + c (2), phephorbides a, peridinin, methyl-chlorophyllide a, fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b allomer, chlorophyll b, chlorophyll a allomer, chlorophyll a, and beta,beta-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.
KeywordHigh-performance Liquid Chromatographic (Hplc) Algae Pigment Chemotaxonomy
Department[Hou, Yansong; Liang, Wei; Zhang, Liping; Cheng, Shuiping; He, Feng; Wu, Zhenbin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Hou, Yansong] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaEngineering ; Environmental ; Environmental Sciences
Funding OrganizationNational Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015]
Indexed BySCI
Funding OrganizationNational Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015] ; National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15508
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiang, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; liangwei02@tsinghua.org.cn, wuzb@ihb.ac.cn
Recommended Citation
GB/T 7714
Hou, Yansong,Liang, Wei,Zhang, Liping,et al. Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA,2011,5(1):84-91.
APA Hou, Yansong.,Liang, Wei.,Zhang, Liping.,Cheng, Shuiping.,He, Feng.,...&liangwei02@tsinghua.org.cn, wuzb@ihb.ac.cn.(2011).Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis.FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA,5(1),84-91.
MLA Hou, Yansong,et al."Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis".FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA 5.1(2011):84-91.
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