IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein
Guan, Ruizhang; Xiong, Jing; Huang, Wenshu; Guo, Songlin; Guan, RZ, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; rzguan@jmu.edu.cn
2011
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN1672-9145
Volume43Issue:1Pages:79-88
AbstractTo develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (OMP) antigen from different bacterial pathogens. In this study, an OMP fragment of 747 bp (named as Omp-G), which was highly conserved in seven Aeromonas OMP sequences from the NCBI database, was amplified by PCR from one Aeromonas sobria strain (B10) and two Aeromonas hydrophila strains (B27 and B33) with the designed specific primers. The sequence was cloned into pGEX-2T (6 x His-tag) vector, expressed in Escherichia coli system, and then the recombinant protein (named as rOmp-G) was purified with nickel chelating affinity chromatography. The purified rOmp-G showed a good immunogenicity in rabbits and well-conserved characteristics in these three pathogens by enzyme-linked immunosorbed assay. Furthermore, the rOmp-G also showed good immunogenicity in eels (Anguilla anguilla) for eliciting significantly increased specific antibodies (P < 0.01), and providing higher protection efficiencies (P < 0.05) after the pathogens challenge. The values of the relative percent survival in eels were 70% and 50% for two A. hydrophila strain challenge, and 75% for A. sobria strain challenge. This is the first report of a potential vaccination in eels that simultaneously provide protectiveness against different Aeromonas pathogens with a conserved partial OMP.
KeywordOuter Membrane Protein Immunogenicity Aeromonas Sobria Aeromonas Hydrophila Anguilla Anguilla
Department[Guan, Ruizhang; Xiong, Jing] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Guan, Ruizhang; Xiong, Jing; Huang, Wenshu; Guo, Songlin] Jimei Univ, Fishery Coll, Xiamen 361021, Peoples R China; [Guan, Ruizhang; Xiong, Jing] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Biophysics
Funding OrganizationMinistry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244]
Indexed BySCI
Language英语
WOS IDWOS:000285628600011
Funding OrganizationMinistry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244] ; Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15504
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuan, RZ, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; rzguan@jmu.edu.cn
Recommended Citation
GB/T 7714
Guan, Ruizhang,Xiong, Jing,Huang, Wenshu,et al. Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(1):79-88.
APA Guan, Ruizhang,Xiong, Jing,Huang, Wenshu,Guo, Songlin,Guan, RZ, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China,&rzguan@jmu.edu.cn.(2011).Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(1),79-88.
MLA Guan, Ruizhang,et al."Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.1(2011):79-88.
Files in This Item:
File Name/Size DocType Version Access License
Enhancement of prote(408KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Guan, Ruizhang]'s Articles
[Xiong, Jing]'s Articles
[Huang, Wenshu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Guan, Ruizhang]'s Articles
[Xiong, Jing]'s Articles
[Huang, Wenshu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Guan, Ruizhang]'s Articles
[Xiong, Jing]'s Articles
[Huang, Wenshu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.