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Shortening the breeding period of transgenic fish using growth hormone levels as an indicator
Zhong, S.; Wang, Y.; Chen, Z.; Lu, P.; Liao, L.; Zhu, Z.; Wang, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; wangyp@ihb.ac.cn
2010
Source PublicationJOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
Volume26Issue:6Pages:904-907
AbstractIn order to shorten the breeding period of fast-growing transgenic fish and reduce the workload of selection, this study introduced a new screening method to detect the growth hormone (GH) serum levels in Cyprinid fish by the combination of ELISA and monoclonal antibody technology. To test the effectiveness of this new method, the serum GH levels and growth rates between transgenic carps and controls were examined; at the same time the conventional PCR method was also employed for comparison. In the F-4-generation, which were the descendents of fast-growing transgenic or normal carps, the serum GH levels between transgenic and control carps were significantly different by ELISA. Transgenic carp growth rates were consistently and significantly higher than in the controls. Compared with the conventional PCR screening that easily resulted in false positive outcomes, the new screening method by ELISA detection directly selected the effectively integrated transgenic fishes, which can remarkably shorten the breeding period of transgenic fish.
Department[Zhong, S.; Wang, Y.; Liao, L.; Zhu, Z.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhong, S.; Chen, Z.; Lu, P.] Wuhan Univ, Basic Med Sch, Dept Genet, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
Funding OrganizationChinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652]
Indexed BySCI
Language英语
WOS IDWOS:000284171500011
Funding OrganizationChinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652] ; Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15498
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; wangyp@ihb.ac.cn
Recommended Citation
GB/T 7714
Zhong, S.,Wang, Y.,Chen, Z.,et al. Shortening the breeding period of transgenic fish using growth hormone levels as an indicator[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2010,26(6):904-907.
APA Zhong, S..,Wang, Y..,Chen, Z..,Lu, P..,Liao, L..,...&wangyp@ihb.ac.cn.(2010).Shortening the breeding period of transgenic fish using growth hormone levels as an indicator.JOURNAL OF APPLIED ICHTHYOLOGY,26(6),904-907.
MLA Zhong, S.,et al."Shortening the breeding period of transgenic fish using growth hormone levels as an indicator".JOURNAL OF APPLIED ICHTHYOLOGY 26.6(2010):904-907.
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