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学科主题: Fisheries; Marine & Freshwater Biology
题名: Shortening the breeding period of transgenic fish using growth hormone levels as an indicator
作者: Zhong, S.1, 2; Wang, Y.1; Chen, Z.2; Lu, P.2; Liao, L.1; Zhu, Z.1
通讯作者: Wang, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; wangyp@ihb.ac.cn
刊名: JOURNAL OF APPLIED ICHTHYOLOGY
发表日期: 2010-12-01
DOI: 10.1111/j.1439-0426.2010.01519.x
卷: 26, 期:6, 页:904-907
收录类别: SCI
文章类型: Article
部门归属: [Zhong, S.; Wang, Y.; Liao, L.; Zhu, Z.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhong, S.; Chen, Z.; Lu, P.] Wuhan Univ, Basic Med Sch, Dept Genet, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652]
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: In order to shorten the breeding period of fast-growing transgenic fish and reduce the workload of selection, this study introduced a new screening method to detect the growth hormone (GH) serum levels in Cyprinid fish by the combination of ELISA and monoclonal antibody technology. To test the effectiveness of this new method, the serum GH levels and growth rates between transgenic carps and controls were examined; at the same time the conventional PCR method was also employed for comparison. In the F-4-generation, which were the descendents of fast-growing transgenic or normal carps, the serum GH levels between transgenic and control carps were significantly different by ELISA. Transgenic carp growth rates were consistently and significantly higher than in the controls. Compared with the conventional PCR screening that easily resulted in false positive outcomes, the new screening method by ELISA detection directly selected the effectively integrated transgenic fishes, which can remarkably shorten the breeding period of transgenic fish.
英文摘要: In order to shorten the breeding period of fast-growing transgenic fish and reduce the workload of selection, this study introduced a new screening method to detect the growth hormone (GH) serum levels in Cyprinid fish by the combination of ELISA and monoclonal antibody technology. To test the effectiveness of this new method, the serum GH levels and growth rates between transgenic carps and controls were examined; at the same time the conventional PCR method was also employed for comparison. In the F-4-generation, which were the descendents of fast-growing transgenic or normal carps, the serum GH levels between transgenic and control carps were significantly different by ELISA. Transgenic carp growth rates were consistently and significantly higher than in the controls. Compared with the conventional PCR screening that easily resulted in false positive outcomes, the new screening method by ELISA detection directly selected the effectively integrated transgenic fishes, which can remarkably shorten the breeding period of transgenic fish.
关键词[WOS]: BODY-COMPOSITION ; CYPRINUS-CARPIO ; GENE ; ENHANCEMENT ; PERFORMANCE ; AQUACULTURE ; TROUT
语种: 英语
WOS记录号: WOS:000284171500011
ISSN号: 0175-8659
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15498
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Basic Med Sch, Dept Genet, Wuhan 430072, Peoples R China

Recommended Citation:
Zhong, S.; Wang, Y.; Chen, Z.; Lu, P.; Liao, L.; Zhu, Z..Shortening the breeding period of transgenic fish using growth hormone levels as an indicator,JOURNAL OF APPLIED ICHTHYOLOGY,2010,26(6):904-907
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