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学科主题: Virology
题名: Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell
作者: Zhu, Ruo-Lin ; Lei, Xiao-Ying ; Ke, Fei ; Yuan, Xiu-Ping ; Zhang, Qi-Ya
通讯作者: Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China ; zhangqy@ihb.ac.cn
关键词: Fish rhabdovirus ; Genome ; Turbot Scophthalmus maximus ; Non-coding region ; Expressed ORF Ps
刊名: VIRUS RESEARCH
发表日期: 2011
卷: 155, 期:2, 页:495-505
收录类别: SCI
部门归属: [Zhu, Ruo-Lin; Lei, Xiao-Ying; Ke, Fei; Yuan, Xiu-Ping; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
资助者: National Major Basic Research Program [2009CB118704, 2010CB126303]; National 863 High Technology Research Foundation of China [2006AA10A402]; National Natural Science Foundation of China [30871938, 31072239]; Agriculture Commonweal Scientific Research Plan [200803013]; Chinese Academy of Sciences [KSCX2-YW-G-075-19]
摘要: Genomic sequence of Scophthalmus maximus rhabdovirus (SMRV) isolated from diseased turbot has been characterized. The complete genome of SMRV comprises 11,492 nucleotides and encodes five typical rhabdovirus genes N, P. M, G and L In addition, two open reading frames (ORF) are predicted overlapping with P gene, one upstream of P and smaller than P (temporarily called Ps), and another in P gene which may encodes a protein similar to the vesicular stomatitis virus C protein. The C ORF is contained within the P ORF. The five typical proteins share the highest sequence identities (48.9%) with the corresponding proteins of rhabdoviruses in genus Vesiculovirus. Phylogenetic analysis of partial L protein sequence indicates that SMRV is close to genus Vesiculovirus. The first 13 nucleotides at the ends of the SMRV genome are absolutely inverse complementarity. The gene junctions between the five genes show conserved polyadenylation signal (CATGA(7)) and intergenic dinucleotide (CT) followed by putative transcription initiation sequence A(A/G)(C/G)A(A/G/T), which are different from known rhabdoviruses. The entire Ps ORF was cloned and expressed, and used to generate polyclonal antibody in mice. One obvious band could be detected in SMRV-infected carp leucocyte cells (CLCs) by anti-Ps/C serum via Western blot, and the subcellular localization of Ps-GFP fusion protein exhibited cytoplasm distribution as multiple punctuate or doughnut shaped foci of uneven size. (C) 2010 Elsevier B.V. All rights reserved.
语种: 英语
WOS记录号: WOS:000287544400014
ISSN号: 0168-1702
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15490
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Zhu, Ruo-Lin; Lei, Xiao-Ying; Ke, Fei; Yuan, Xiu-Ping; Zhang, Qi-Ya.Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell,VIRUS RESEARCH,2011,155(2):495-505
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