IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains
Chang, Mingxian; Wang, Tiehui; Nie, Pin; Zou, Jun; Secombes, Christopher J.; Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; c.secombes@abdn.ac.uk
2011
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume30Issue:1Pages:118-127
AbstractNucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordRainbow Trout Nod2 Proinflammatory Cytokine Apoptosis
Department[Chang, Mingxian; Wang, Tiehui; Zou, Jun; Secombes, Christopher J.] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; [Chang, Mingxian; Nie, Pin] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Chang, Mingxian; Nie, Pin] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU
Indexed BySCI
Language英语
WOS IDWOS:000286905800015
Funding OrganizationNational Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU ; National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15486
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorSecombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; c.secombes@abdn.ac.uk
Recommended Citation
GB/T 7714
Chang, Mingxian,Wang, Tiehui,Nie, Pin,et al. Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):118-127.
APA Chang, Mingxian.,Wang, Tiehui.,Nie, Pin.,Zou, Jun.,Secombes, Christopher J..,...&c.secombes@abdn.ac.uk.(2011).Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains.FISH & SHELLFISH IMMUNOLOGY,30(1),118-127.
MLA Chang, Mingxian,et al."Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):118-127.
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