中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
学科主题: Biotechnology & Applied Microbiology; Genetics & Heredity
题名: The rapid generation of chimerical genes expanding protein diversity in zebrafish
作者: Fu, Beide1, 2; Chen, Ming1, 2; Zou, Ming1, 2; Long, Manyuan3; He, Shunping1
通讯作者: He, SP, Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China ; heshunping@gmail.com
刊名: BMC GENOMICS
发表日期: 2010-11-24
DOI: 10.1186/1471-2164-11-657
卷: 11, 页:657-
收录类别: SCI
文章类型: Article
部门归属: [Fu, Beide; Chen, Ming; Zou, Ming; He, Shunping] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Fu, Beide; Chen, Ming; Zou, Ming] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China; [Long, Manyuan] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Natural Science Foundation on China [2007CB411601]
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
摘要: Background: Variation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin of new genes is gene duplication. Retroposition, as a special type of gene duplication-the RNA-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome. Results: Here we screened the zebrafish genome for identification of retrocopies and new chimerical retrogenes and investigated their origination and evolution. We identified 652 retrocopies, of which 440 are intact retrogenes and 212 are pseudogenes. Retrocopies have long been considered evolutionary dead ends without functional significance due to the presumption that retrocopies lack the regulatory element needed for expression. However, 437 transcribed retrocopies were identified from all of the retrocopies. This discovery combined with the substitution analysis suggested that the majority of all retrocopies are subject to negative selection, indicating that most of the retrocopies may be functional retrogenes. Moreover, we found that 95 chimerical retrogenes had recruited new sequences from neighboring genomic regions that formed de novo splice sites, thus generating new intron-containing chimeric genes. Based on our analysis of 38 pairs of orthologs between Cyprinus carpio and Danio rerio, we found that the synonymous substitution rate of zebrafish genes is 4.13x10(-9) substitution per silent site per year. We also found 10 chimerical retrogenes that were created in the last 10 million years, which is 7.14 times the rate of 0.14 chimerical retrogenes per million years in the primate lineage toward human and 6.25 times the rate of 0.16 chimerical genes per million years in Drosophila. This is among the most rapid rates of generation of chimerical genes, just next to the rice. Conclusion: There is compelling evidence that much of the extensive transcriptional activity of retrogenes does not represent transcriptional "noise" but indicates the functionality of these retrogenes. Our results indicate that retroposition created a large amount of new genes in the zebrafish genome, which has contributed significantly to the evolution of the fish genome.
英文摘要: Background: Variation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin of new genes is gene duplication. Retroposition, as a special type of gene duplication-the RNA-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome.
关键词[WOS]: HUMAN GENOME ; PROCESSED PSEUDOGENES ; RETROPOSED GENE ; EVOLUTION ; DROSOPHILA ; ORIGIN ; ELEMENTS ; RETROGENES ; YOUNG ; RETROTRANSPOSONS
语种: 英语
WOS记录号: WOS:000285253800002
ISSN号: 1471-2164
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/15462
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
The rapid generation of chimerical genes expanding protein diversity in zebrafish.pdf(379KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA

Recommended Citation:
Fu, Beide; Chen, Ming; Zou, Ming; Long, Manyuan; He, Shunping.The rapid generation of chimerical genes expanding protein diversity in zebrafish,BMC GENOMICS,2010,11():657-
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Fu, Beide]'s Articles
[Chen, Ming]'s Articles
[Zou, Ming]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Fu, Beide]‘s Articles
[Chen, Ming]‘s Articles
[Zou, Ming]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: The rapid generation of chimerical genes expanding protein diversity in zebrafish.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace