IHB OpenIR  > 会议论文
微囊藻毒素直接竞争ELISA的检测法的初步建立 
雷腊梅; 宋立荣; 吴英松
2001
Conference Name第七届海峡两岸环境保护学术研讨会
Source Publication21世纪可持续发展之环境保护论文集 
Pages180-183
Conference Place武汉
Publication Place武汉大学出版社
Publisher武汉大学出版社
Department中国科学院水生生物研究所(武汉)
Abstract收集滇池微囊藻水华,烘干,用5﹪的乙酸抽提微囊藻毒素,最后用HPLC分离制备毒素,经分析,毒素纯度为50﹪左右.将制备所得的微囊藻毒素LR通过EDA与BSA连接作为抗菌素原免疫昆明鼠,应用直接竞争ELISA法检测待测样品.藻细胞的破碎采用简单的沸水浴法.结果显示,引起50﹪抑制率标准品浓度为1.8ng/mL.线性范围为0.1~10ng/mL.最低检出量为0.2ng/mL,即50pg.共检测了23个样品,其中20个样品含有毒素. 
Keyword水体环境 蓝藻水华 微囊藻毒素 抗体 Elisa
Funding Organization武汉大学
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15385
Collection会议论文
Recommended Citation
GB/T 7714
雷腊梅,宋立荣,吴英松. 微囊藻毒素直接竞争ELISA的检测法的初步建立 [C]. 武汉大学出版社:武汉大学出版社,2001:180-183.
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