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半眉虫(Hemiophrys sp.)的系统发育研究
朱飞云; 余育和; 沈蕴芬
Source Publication中国原生动物学学会第十一次学术讨论会论文摘要汇编
Abstract<正> 采自东湖的一种常见半眉虫(Hemiophrys sp.),以微吸管逐个挑出无菌水清洗后置于室温饥饿8小时以上。离心收集此半眉虫。以酚—氯仿法抽提其基因组DNA。用Medlin在1988发表的纤毛虫通用的核糖体小亚基RNA基因(ssrDNA)全序列的扩增引物进行基因扩增,引物序列分别为5’TGATCCTTCTGCAGGTTCACCT 3’和 5’AACCTGGTTGATCCTGCCAG 3’。由克隆、测序得到的结果可知半眉虫的核糖体小亚基RNA基因序列的全长为1636bp。其中GC的含量为42.54%。
Document Type会议论文
Recommended Citation
GB/T 7714
朱飞云,余育和,沈蕴芬. 半眉虫(Hemiophrys sp.)的系统发育研究[C],2002.
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