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波豆科鞭毛虫ss rRNA分子系统发育研究
谷习文; 余育和; 沈蕴芬
2002
Source Publication中国原生动物学学会第十一次学术讨论会论文摘要汇编
Abstract<正> 作者2001年5~6月份于武昌东湖采集水样中分离出一种鞭毛虫;鉴定为Bodocurvifilus。克隆培养(%0.125Ceraphy1;加入细菌)后;利用活体和DAPI染色技术进行了其一般形态学及其他显微结构的研究;随后进一步研究了其亚显微结构。上述研究确认该物种。波豆属共有200多个已命名的种;检索GeneBank发现该属有五个种的SSUrRNA(核糖体小亚基RNA)序列已被测定;Bodo curvifilus不在其中。因此;作者拟对Bodo Curvifilus进行 SSUrRNA测序。利用酚抽提法提取了Bodo curvifilus的基因组DNA;然后利用特异性引物扩增其核糖体小亚基RNA;测得其ss RNA的全序列共2100bp。GeneBank己有的波豆类小亚基RNA序列分属于七个属;共17个种。
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15016
Collection会议论文
Recommended Citation
GB/T 7714
谷习文,余育和,沈蕴芬. 波豆科鞭毛虫ss rRNA分子系统发育研究[C],2002.
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