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累枝虫属(Genus Epistylis)5SrRNA基因的序列测定及其与系统发育的关系
程兴国; 陈乃清; 易清明; 余育和; 沈韫芬
2002
Source Publication中国原生动物学学会第十一次学术讨论会论文摘要汇编
Abstract<正> 5SrRNA基因在生物中普遍存在;其分子小;长度仅为120核苷酸;且相当保守。本文的目的就是试图通过5S rRNA基因的序列来确定不同原生动物之间的亲缘关系。在为数众多的原生动物中;目前仅30种已经确定了其5SrRNA基因序列。据此;我们设计并合成了一对引物:Forward primer:5’-CGGCTTAAGGCTTTGGTTAG-3’;reverseprimer:5’-AACTTCGGAGTTCGGATGG-3’.随后;我们以褶累枝虫基因组DNA为模板;进行PCR反应;将扩增产物克隆至载体PGEM-3Zf(+);并测定此克隆中的外源DNA
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/15002
Collection会议论文
Recommended Citation
GB/T 7714
程兴国,陈乃清,易清明,等. 累枝虫属(Genus Epistylis)5SrRNA基因的序列测定及其与系统发育的关系[C],2002.
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