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银鲫和彩鲫卵母细胞c-型凝集素的鉴定、特征及其功能分析
桂建芳; 董彩华; 杨书婷; 杨仲安
2003
Source Publication中国的遗传学研究——中国遗传学会第七次代表大会暨学术讨论会论文摘要汇编
Abstract<正> 借鉴非细胞体系(cell-free systems)的研究方法;制备了雌核发育银鲫和两性生殖彩鲫成熟卵子的抽提液;采用不同电泳系统对银鲫和彩鲫卵子抽提液中的蛋白成分进行比较分析。结果表明;虽然抽提液中蛋白质成分多而复杂;在SDS-PAGE胶上分辨不出相互之间的差异;但在非变性梯度凝聚电泳图谱上则各存在有一组差异的蛋白谱带;银鲫差异蛋白谱带的迁移率快于彩鲫差异蛋白谱带。后经P11和DE52离子交换柱以及CHT亲和柱层析等步骤;纯化得到一个银鲫的差异蛋白。该蛋白经SDS-PAGE胶电泳和考马斯亮蓝显色表现为一条带;在经2D-PAGE胶电泳也表现为一个单点;表明该蛋白已被纯化;其分子量约为22kDa。纯化的蛋白经氨基酸测序确定了其12个氨基酸肽段序列;用据此设计的引物;从银鲫卵母细胞cDNA文库中克隆出了其全长cDNA;并从彩鲫卵母细胞cDNA文库中也克隆出了与该差异蛋白基因同
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14980
Collection会议论文
Recommended Citation
GB/T 7714
桂建芳,董彩华,杨书婷,等. 银鲫和彩鲫卵母细胞c-型凝集素的鉴定、特征及其功能分析[C],2003.
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