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湖北钉螺细胞原代培养的初步研究
彭延; 蒋明森; 钟沁萍; 桂建芳; 董惠芬
2004
Source Publication中国动物学会全国第九次寄生虫学学术讨论会论文摘要集
Abstract<正> 目的研究湖北钉螺组织细胞的原代培养。方法无菌处理并解剖钉螺成螺及螺胚,分别获得软体、肝脏、外套膜及胚胎组织。将软体、肝脏及胚胎组织弄碎,用0.25%胰蛋白酶和0.02%EDTA混合液4℃下消化,消化所得细胞直接接种;将外套膜按组织块贴壁法接种。培养液为1/2 RPMI-1640含20%小牛血清附加常量抗生素(青霉素100IU/ml,链霉素100μg/ml),培养温度为27~28℃,pH值为7.2~7.4。结果重复培养11次钉螺的体细胞、肝脏细胞均遭污染。组织块贴壁法培养的外套膜组织,游离出
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14924
Collection会议论文
Recommended Citation
GB/T 7714
彭延,蒋明森,钟沁萍,等. 湖北钉螺细胞原代培养的初步研究[C],2004.
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