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牙鲆精氨酸转甲基酶基因的克隆与原核表达
董彩文; 张奇亚; 杜昌生; 李正秋; 桂建芳
2004
Source Publication湖北省遗传学会第七次代表大会暨学术讨论会论文摘要集
Abstract<正> 蛋白质的甲基化如同磷酸化;在RNA加工中;涉及到翻译后的调控;mRNA成熟;转移和稳定;也是细胞因子受体的一种重要的信号传导机制。根据抑制性差减杂交方法建立的牙鲆正常囊胚细胞和受灭活弹状病毒诱导的囊胚细胞之间的差减文库;利用斑点杂交方法筛选出牙鲆精氨酸转甲基酶基因的480bp大小片段;根据该序列设计合成特异引物;应用RACE-PCR方法分别扩增该基因的5’末端和3’末端;经过序列拼接得到全长基因。序列分析表明:所克隆的牙鲆精氨酸转甲基酶基因cDNA长1671bp;编码341个氨基酸。与已
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14870
Collection会议论文
Recommended Citation
GB/T 7714
董彩文,张奇亚,杜昌生,等. 牙鲆精氨酸转甲基酶基因的克隆与原核表达[C],2004.
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