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斜带石斑鱼垂体SMART cDNA文库的构建及EST分析
李创举; 周莉; 桂建芳
2007
Source Publication湖北省遗传学会、江西省遗传学会2006年学术年会暨学术讨论会论文摘要集
Abstract<正>斜带石斑鱼是重要的海产经济鱼类;在其个体发育中存在先雌后雄的天然性反转现象。下丘脑-垂体-性腺轴是调节鱼类生长和生殖的中心轴;我们选取斜带石斑鱼垂体作为研究对象。首先;合成了雌、雄性石斑鱼垂体SMART cDNA;将其连入pGEM-T载体;再转化大肠杆菌; 用PCR方法筛选出插入片段为400bp以上的阳性克隆进行测序。雌、雄石斑鱼垂体cDNA库中
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14738
Collection会议论文
Recommended Citation
GB/T 7714
李创举,周莉,桂建芳. 斜带石斑鱼垂体SMART cDNA文库的构建及EST分析[C],2007.
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