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草鱼出血病病毒rna干扰的抗病研究
陈芸; 廖莎; 汪亚平; 胡炜; 邹均; 朱作言
2007
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Source Publication遗传学进步与人口健康高峰论坛论文集
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Abstract<正>以草鱼出血病病毒(GCRV)外壳蛋白基因 VP7为靶基因;选择3个不同的 siRNA 位点;构建了3个草鱼 H1基因启动子驱动的 shRNA 表达载体 pH1siGCRV(X)-CMVeGFP 及其对照表达载体 pH1shHK-CMVeGFP。草鱼肾细胞(CIK) 的感染实验结果证实;pH1shGCRV2-CMVeGFP 在细胞水平上具有较强的病毒抑制作用;抑制率达82%;以 pH1shGCRV2-CMVeGFP 和 pH1shHK-CMVeGFP 为外源基因;采用显微注射方法;获得转基因稀有(鱼句)鲫 P0代群体;3
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14726
Collection会议论文
Recommended Citation
GB/T 7714
陈芸,廖莎,汪亚平,等. 草鱼出血病病毒rna干扰的抗病研究[C],2007.
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