IHB OpenIR  > 会议论文
银鲫精巢组织cDNA文库的构建
王达; 周莉; 彭金霞; 桂建芳
2008
Source Publication中国海洋湖沼学会第九次全国会员代表大会暨学术研讨会论文摘要汇编
Abstract<正>三倍体银鲫是同时具有雌核生殖和两性生殖的特殊三倍体鱼类,在前期的研究中,我们从克隆 D 和克隆 A 交配的子代中,通过几代雌核发育获得了一个特殊的雌核发育全雄突变系。为揭示该突变系产生的分子机制及其银鲫性别决定和分化的分子机制,本研究运用 SMART 技术构建了银鲫 DA-D 全雄突变系精巢组织全长 cDNA 文库。首先用 Trizol reagent(GIBCO BRL) 提取银鲫精巢总 RNA,用 Oligotex mRNA Kits(QIAGEN)分离纯化 mRNA,接着用 SMART
Language中文
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14656
Collection会议论文
Recommended Citation
GB/T 7714
王达,周莉,彭金霞,等. 银鲫精巢组织cDNA文库的构建[C],2008.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[王达]'s Articles
[周莉]'s Articles
[彭金霞]'s Articles
Baidu academic
Similar articles in Baidu academic
[王达]'s Articles
[周莉]'s Articles
[彭金霞]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[王达]'s Articles
[周莉]'s Articles
[彭金霞]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.