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中华鳖免疫球蛋白j链基因的克隆与表达
徐镇; 昌明先; 齐志涛; 黄贝; 聂品
2009
Conference Name-
Source Publication2008年中国水产学会学术年会论文摘要集
Conference Date-
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Abstract用RACE-PCR方法扩增出中华鳖Trionyx sinensis免疫球蛋白J链基因全长cDNA。其cDNA全长为2347 bp,包含5|非编码区30 bp,3||非编码区1,834 bp,开放阅读框480 bp,编码160个氨基酸。氨基酸序列对比分析表明中华鳖免疫球蛋白J链基因与红耳龟的相似性高达80.7%,与鸡、人、牛、鼠、兔、爪蟾以及鳐的相似性分别为71.3%、58.5%、59.6%、60.8%、50.9%、57.6%和35.8%。系统发育分析表明,中华鳖免疫球蛋白J链基因与红耳龟的J链基因聚为一枝。RT-PCR和免疫印记显示中华鳖免疫球蛋白J链在mRNA和蛋白水平具有相似的表达模式,组成性表达于心、肝、脾、肾、胃、肠以及血液中。免疫组化分析揭示健康中华鳖的肝、脾、肾和肠中有J链蛋白的存在,表明中华鳖的J链基因可能具有与高等脊椎动物一样的促进免疫球蛋白分泌表达的功能。实时荧光定量PCR分析显示经灭活的嗜水气单胞菌Aeromonas hydrophila T4诱导后J链基因分别在脾、肾和血液中有显著上调,表明中华鳖J链基因在免疫应答中起重要作用。
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14550
Collection会议论文
Recommended Citation
GB/T 7714
徐镇,昌明先,齐志涛,等. 中华鳖免疫球蛋白j链基因的克隆与表达[C],2009.
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