Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)

	Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
	Guan, Bo (1); Ma, Hong (3); Wang, Yaping (1); Hu, Yuanlei (3); Lin, Zhongping (3); Zhu, Zuoyan (1); Hu, Wei (1); Hu,W. (huwei@ihb.ac.cn); Hu, Y.(huyl@pku.edu.cn)
	2010
Source Publication	Marine Biotechnology
ISSN	1436-2228
Pages	1-9
Abstract	Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish. © 2010 Springer Science+Business Media, LLC.
Keyword	Vitreoscilla Hemoglobin (Vhb) Hypoxia Zebrafish (Danio Rerio) Transgenic Fish
Department	(1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; (2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China; (3) College of Life Sciences, Peking University, Beijing, 100871, China
Indexed By	EI
Language	英语
Document Type	期刊论文
Identifier	http://ir.ihb.ac.cn/handle/342005/14460
Collection	鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding Author	Hu,W. (huwei@ihb.ac.cn); Hu, Y.(huyl@pku.edu.cn)
Recommended Citation GB/T 7714	Guan, Bo ,Ma, Hong ,Wang, Yaping ,et al. Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)[J]. Marine Biotechnology,2010:1-9.
APA	Guan, Bo .,Ma, Hong .,Wang, Yaping .,Hu, Yuanlei .,Lin, Zhongping .,...&Hu, Y..(2010).Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).Marine Biotechnology,1-9.
MLA	Guan, Bo ,et al."Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)".Marine Biotechnology (2010):1-9.

Files in This Item:
File Name/Size	DocType	Version	Access	License
Vitreoscilla Hemoglo（236KB）			开放获取	--	View Application Full Text

File name:	Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院水生生物研究所机构知识库