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Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
Guan, Bo (1); Ma, Hong (3); Wang, Yaping (1); Hu, Yuanlei (3); Lin, Zhongping (3); Zhu, Zuoyan (1); Hu, Wei (1); Hu,W. (huwei@ihb.ac.cn); Hu, Y.(huyl@pku.edu.cn)
2010
Source PublicationMarine Biotechnology
ISSN1436-2228
Pages1-9
AbstractAquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish. © 2010 Springer Science+Business Media, LLC.
KeywordVitreoscilla Hemoglobin (Vhb) Hypoxia Zebrafish (Danio Rerio) Transgenic Fish
Department(1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; (2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China; (3) College of Life Sciences, Peking University, Beijing, 100871, China
Indexed ByEI
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14460
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu,W. (huwei@ihb.ac.cn); Hu, Y.(huyl@pku.edu.cn)
Recommended Citation
GB/T 7714
Guan, Bo ,Ma, Hong ,Wang, Yaping ,et al. Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)[J]. Marine Biotechnology,2010:1-9.
APA Guan, Bo .,Ma, Hong .,Wang, Yaping .,Hu, Yuanlei .,Lin, Zhongping .,...&Hu, Y..(2010).Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).Marine Biotechnology,1-9.
MLA Guan, Bo ,et al."Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)".Marine Biotechnology (2010):1-9.
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