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题名: Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China
作者: Lin, Shen1, 4; Shen, Jianzhong2; Liu, Yang1, 4; Wu, Xingqiang1, 4; Liu, Qigen3; Li, Renhui1
通讯作者: Li, R.(reli@ihb.ac.cn)
关键词: Lake Ulungur ; Microcystis ; Real-time PCR ; HPLC ; Microcystin ; Mcy gene ; Brackish lake
刊名: ENVIRONMENTAL MONITORING AND ASSESSMENT
发表日期: 2011-04-01
DOI: 10.1007/s10661-010-1500-x
卷: 175, 期:1-4, 页:139-150
收录类别: SCI
文章类型: Article
部门归属: (1) Key Laboratory of Aquatic Biodiversity and Conservation Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; (2) Fishery College, Huazhong Agricultural University, Wuhan, 430070, China; (3) College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, 200090, China; (4) Graduate University of Chinese Academy of Sciences, Beijing, 100039, China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: The distribution and diversity of the waterbloom-forming cyanobacteria, mainly including Microcystis species, were investigated using molecular approaches in Lake Ulungur, an increasingly eutrophic and brackish lake located in Xinjiang, China. Real-time PCR analyses showed the abundance of Microcystis 16S rDNA gene copies in the Ulungur Lake is low, at 7.2 × 104 copies L - 1 averagely and 4.5 × 105 copies L - 1 at maximum. Two Microcystis species, M. aeruginosa (Ku¨tzing) Lemmermann and Microcystis wesenbergii (Koma´rek) Koma´rek were, for the first time, reported in this lake. The mcyA gene-specific PCR determination on the isolates of Microcystis showed that the M. aeruginosa strains are all mcyA-containing genotypes, while M. wesenbergii are non-mcyA-containing ones. The microcystin contents of the toxic M. aeruginosa strains were shown to be lower than those of the Microcystis strains isolated from other eutrophic lakes in China. Phylogenetic analyses based on 16S rRNA and rpoC1 genes showed that the Microcystis strains isolated from the Ulungur Lake were not genetically divergent from those isolated in the other freshwaters. Such an investigation would contribute to the knowledge on the bloom-forming cyanobacteria of the increasingly eutrophic and saline lakes in the desert area. © 2010 Springer Science+Business Media B.V.
英文摘要: The distribution and diversity of the waterbloom-forming cyanobacteria, mainly including Microcystis species, were investigated using molecular approaches in Lake Ulungur, an increasingly eutrophic and brackish lake located in Xinjiang, China. Real-time PCR analyses showed the abundance of Microcystis 16S rDNA gene copies in the Ulungur Lake is low, at 7.2 x 10(4) copies L (-aEuro parts per thousand 1) averagely and 4.5 x 10(5) copies L (-aEuro parts per thousand 1) at maximum. Two Microcystis species, M. aeruginosa (Kutzing) Lemmermann and Microcystis wesenbergii (Komarek) Komarek were, for the first time, reported in this lake. The mcyA gene-specific PCR determination on the isolates of Microcystis showed that the M. aeruginosa strains are all mcyA-containing genotypes, while M. wesenbergii are non-mcyA-containing ones. The microcystin contents of the toxic M. aeruginosa strains were shown to be lower than those of the Microcystis strains isolated from other eutrophic lakes in China. Phylogenetic analyses based on 16S rRNA and rpoC1 genes showed that the Microcystis strains isolated from the Ulungur Lake were not genetically divergent from those isolated in the other freshwaters. Such an investigation would contribute to the knowledge on the bloom-forming cyanobacteria of the increasingly eutrophic and saline lakes in the desert area.
关键词[WOS]: REAL-TIME PCR ; GENUS MICROCYSTIS ; BLOOMS ; BIOSYNTHESIS ; EVOLUTION ; SEQUENCES ; GENOTYPES ; WATER
语种: 英语
WOS记录号: WOS:000288026600012
ISSN号: 0167-6369
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/14444
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 200090, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

Recommended Citation:
Lin, Shen (1); Shen, Jianzhong (2); Liu, Yang (1); Wu, Xingqiang (1); Liu, Qigen (3); Li, Renhui (1) .Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China,Environmental Monitoring and Assessment,2010,():1-12
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