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Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
Miao, Wei1,2; Xiong, Jie2; Bowen, Josephine1; Wang, Wei1,3; Liu, Yifan4; Braguinets, Olga5; Grigull, Jorg6; Pearlman, Ronald E.5; Orias, Eduardo7; Gorovsky, Martin A.1; Miao, W, Univ Rochester, Dept Biol, Rochester, NY 14627 USA,miaowei530@yeah.net
2009-02-10
Source PublicationPLOS ONE
ISSN1932-6203
Volume4Issue:2Pages:e4429
AbstractBackground: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression. Methodology/Principal Findings: A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation. Conclusions/Significance: Of the,27,000 predicted open reading frames, transcripts homologous to only,5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels >5x corrected background and 95 genes are expressed at >250x corrected background in all stages. Transcripts homologous to 91 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, while 90 are specifically expressed and 616 are up-regulated during starvation. Strikingly, transcripts homologous to 1068 predicted genes are specifically expressed and 1753 are significantly up-regulated during conjugation. The patterns of gene expression during conjugation correlate well with the developmental stages of meiosis, nuclear differentiation and DNA elimination. The relationship between gene expression and chromosome fragmentation is analyzed. Genes encoding proteins known to interact or to function in complexes show similar expression patterns, indicating that co-ordinate expression with putative genes of known function can identify genes with related functions. New candidate genes associated with the RNAi-like process of DNA elimination and with meiosis are identified and the late stages of conjugation are shown to be characterized by specific expression of an unexpectedly large and diverse number of genes not involved in nuclear functions.; Background: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression.
SubtypeArticle
Department[Miao, Wei; Bowen, Josephine; Wang, Wei; Gorovsky, Martin A.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA; [Miao, Wei; Xiong, Jie] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Wang, Wei] Shanxi Univ, Inst Biotechnol, Taiyuan, Peoples R China; [Liu, Yifan] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA; [Braguinets, Olga; Pearlman, Ronald E.] York Univ, Dept Biol, N York, ON M3J 1P3, Canada; [Grigull, Jorg] York Univ, Dept Math & Stat, N York, ON M3J 1P3, Canada; [Orias, Eduardo] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA
Subject AreaBiology ; Multidisciplinary Sciences
DOI10.1371/journal.pone.0004429
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000265484000015
Citation statistics
Cited Times:123[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14438
Collection期刊论文
Corresponding AuthorMiao, W, Univ Rochester, Dept Biol, Rochester, NY 14627 USA,miaowei530@yeah.net
Affiliation1.Univ Rochester, Dept Biol, Rochester, NY 14627 USA
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Shanxi Univ, Inst Biotechnol, Taiyuan, Peoples R China
4.Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA
5.York Univ, Dept Biol, N York, ON M3J 1P3, Canada
6.York Univ, Dept Math & Stat, N York, ON M3J 1P3, Canada
7.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA
Recommended Citation
GB/T 7714
Miao, Wei,Xiong, Jie,Bowen, Josephine,et al. Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle[J]. PLOS ONE,2009,4(2):e4429.
APA Miao, Wei.,Xiong, Jie.,Bowen, Josephine.,Wang, Wei.,Liu, Yifan.,...&Miao, W, Univ Rochester, Dept Biol, Rochester, NY 14627 USA,miaowei530@yeah.net.(2009).Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle.PLOS ONE,4(2),e4429.
MLA Miao, Wei,et al."Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle".PLOS ONE 4.2(2009):e4429.
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