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学科主题: Biology; Multidisciplinary Sciences
题名: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
作者: Miao, Wei1, 2; Xiong, Jie2; Bowen, Josephine1; Wang, Wei1, 3; Liu, Yifan4; Braguinets, Olga5; Grigull, Jorg6; Pearlman, Ronald E.5; Orias, Eduardo7; Gorovsky, Martin A.1
通讯作者: Miao, W, Univ Rochester, Dept Biol, Rochester, NY 14627 USA,miaowei530@yeah.net
刊名: PLOS ONE
发表日期: 2009-02-10
DOI: 10.1371/journal.pone.0004429
卷: 4, 期:2, 页:e4429
收录类别: SCI
文章类型: Article
部门归属: [Miao, Wei; Bowen, Josephine; Wang, Wei; Gorovsky, Martin A.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA; [Miao, Wei; Xiong, Jie] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Wang, Wei] Shanxi Univ, Inst Biotechnol, Taiyuan, Peoples R China; [Liu, Yifan] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA; [Braguinets, Olga; Pearlman, Ronald E.] York Univ, Dept Biol, N York, ON M3J 1P3, Canada; [Grigull, Jorg] York Univ, Dept Math & Stat, N York, ON M3J 1P3, Canada; [Orias, Eduardo] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Background: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression. Methodology/Principal Findings: A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation. Conclusions/Significance: Of the,27,000 predicted open reading frames, transcripts homologous to only,5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels >5x corrected background and 95 genes are expressed at >250x corrected background in all stages. Transcripts homologous to 91 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, while 90 are specifically expressed and 616 are up-regulated during starvation. Strikingly, transcripts homologous to 1068 predicted genes are specifically expressed and 1753 are significantly up-regulated during conjugation. The patterns of gene expression during conjugation correlate well with the developmental stages of meiosis, nuclear differentiation and DNA elimination. The relationship between gene expression and chromosome fragmentation is analyzed. Genes encoding proteins known to interact or to function in complexes show similar expression patterns, indicating that co-ordinate expression with putative genes of known function can identify genes with related functions. New candidate genes associated with the RNAi-like process of DNA elimination and with meiosis are identified and the late stages of conjugation are shown to be characterized by specific expression of an unexpectedly large and diverse number of genes not involved in nuclear functions.
英文摘要: Background: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression.
语种: 英语
WOS记录号: WOS:000265484000015
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/14438
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Univ Rochester, Dept Biol, Rochester, NY 14627 USA
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Shanxi Univ, Inst Biotechnol, Taiyuan, Peoples R China
4.Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA
5.York Univ, Dept Biol, N York, ON M3J 1P3, Canada
6.York Univ, Dept Math & Stat, N York, ON M3J 1P3, Canada
7.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA

Recommended Citation:
Miao, Wei; Xiong, Jie; Bowen, Josephine; Wang, Wei; Liu, Yifan; Braguinets, Olga; Grigull, Jorg; Pearlman, Ronald E.; Orias, Eduardo; Gorovsky, Martin A..Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle,PLOS ONE,2009,4(2):e4429
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