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A colorimetric assay for screening microcystin class compounds in aquatic systems
Bryan S,.F. Wong; Paul K.S. Lain; Lihong Xu; Yongyuan Zhang; Bruce J. Richardson; Wong, BSF, City Univ Hong Kong, Ctr Environm Sci & Technol, Tat Chee Ave, Hong Kong, Peoples R China
1999
Source PublicationCHEMOSPHERE
Volume38Issue:5Pages:1113-1122
AbstractSecondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins, These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a P-32-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow p-nitrophenol using p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. (C) 1998 Elsevier Science Ltd. All rights reserved.
KeywordPhosphatase Inhibition Assay
DepartmentCity Univ Hong Kong, Ctr Environm Sci & Technol, Hong Kong, Peoples R China; City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Subject AreaEnvironmental Sciences
Indexed BySCI
Language英语
WOS IDWOS:000078194100015
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14404
Collection期刊论文
Corresponding AuthorWong, BSF, City Univ Hong Kong, Ctr Environm Sci & Technol, Tat Chee Ave, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Bryan S,.F. Wong,Paul K.S. Lain,Lihong Xu,et al. A colorimetric assay for screening microcystin class compounds in aquatic systems[J]. CHEMOSPHERE,1999,38(5):1113-1122.
APA Bryan S,.F. Wong,Paul K.S. Lain,Lihong Xu,Yongyuan Zhang,Bruce J. Richardson,&Wong, BSF, City Univ Hong Kong, Ctr Environm Sci & Technol, Tat Chee Ave, Hong Kong, Peoples R China.(1999).A colorimetric assay for screening microcystin class compounds in aquatic systems.CHEMOSPHERE,38(5),1113-1122.
MLA Bryan S,.F. Wong,et al."A colorimetric assay for screening microcystin class compounds in aquatic systems".CHEMOSPHERE 38.5(1999):1113-1122.
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