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学科主题: Environmental Sciences
题名: A colorimetric assay for screening microcystin class compounds in aquatic systems
作者: Wong, BSF; Lam, PKS; Xu, LH; Zhang, YY; Richardson, BJ
通讯作者: Wong, BSF, City Univ Hong Kong, Ctr Environm Sci & Technol, Tat Chee Ave, Hong Kong, Peoples R China
关键词: PHOSPHATASE INHIBITION ASSAY
刊名: CHEMOSPHERE
发表日期: 1999-02-01
卷: 38, 期:5, 页:1113-1122
收录类别: SCI
文章类型: Article
部门归属: City Univ Hong Kong, Ctr Environm Sci & Technol, Hong Kong, Peoples R China; City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins, These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a P-32-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow p-nitrophenol using p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. (C) 1998 Elsevier Science Ltd. All rights reserved.
英文摘要: Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins, These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a P-32-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow p-nitrophenol using p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. (C) 1998 Elsevier Science Ltd. All rights reserved.
关键词[WOS]: PHOSPHATASE INHIBITION ASSAY ; OKADAIC ACID ; PROTEIN ; WATER ; CYANOBACTERIA ; PURIFICATION ; AERUGINOSA ; TOXINS ; LR
语种: 英语
WOS记录号: WOS:000078194100015
ISSN号: 0045-6535
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/14404
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.City Univ Hong Kong, Ctr Environm Sci & Technol, Hong Kong, Peoples R China
2.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

Recommended Citation:
.A colorimetric assay for screening microcystin class compounds in aquatic systems,CHEMOSPHERE,1999,38(5):1113-1122
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