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题名: Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
作者: Li Q(李琼) ; Tang Q(唐蜻) ; Xu XD(徐旭东) ; Gao H(高宏)
关键词: cyanobacteria
刊名: Chinese Journal of Oceanology and Limnology
发表日期: 2010
期: 6
收录类别: SCI
部门归属: State Key Laboratory for Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences;Graduate School of the Chinese Academy of Sciences;
资助者: Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001)
摘要: Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp.PCC 7120 and several other non-aggregating species.Mass culture and harvest of such species are more energy consuming relative to aggregating species.To establish a gene transfer system for aggregating species,we tested many species of Anabaena and Nostoc,and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system.To promote biodegradation of organophosphorus pollutants in aquatic environments,we introduced a plasmid containing the organophosphorus-degradation gene(opd) into Anabaena sp.PCC 7120 and Nostoc muscorum FACHB244 by conjugation.The opd gene was driven by a strong promoter,PpsbA.From both species,we obtained transgenic strains having organophosphorus-degradation activities.At 25°C,the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0.163±0.001 and 0.289±0.042 unit/μg Chl a,respectively.However,most colonies resulting from the gene transfer showed no activity.PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies.Expression of the green fluorescent protein gene from the same promoter in Anabaena sp.PCC 7120 showed similar results.These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic engineering of Anabaena and Nostoc species.For the first time,we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium.The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites.
语种: 英语
ISSN号: 0254-4059
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/14252
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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Recommended Citation:
李琼;唐蜻;徐旭东;高宏;.Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria,Chinese Journal of Oceanology and Limnology,2010,(6):
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