IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
Li Q(李琼); Tang Q(唐蜻); Xu XD(徐旭东); Gao H(高宏)
2010
Source PublicationChinese Journal of Oceanology and Limnology
ISSN0254-4059
Issue6
AbstractGenetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp.PCC 7120 and several other non-aggregating species.Mass culture and harvest of such species are more energy consuming relative to aggregating species.To establish a gene transfer system for aggregating species,we tested many species of Anabaena and Nostoc,and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system.To promote biodegradation of organophosphorus pollutants in aquatic environments,we introduced a plasmid containing the organophosphorus-degradation gene(opd) into Anabaena sp.PCC 7120 and Nostoc muscorum FACHB244 by conjugation.The opd gene was driven by a strong promoter,PpsbA.From both species,we obtained transgenic strains having organophosphorus-degradation activities.At 25°C,the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0.163±0.001 and 0.289±0.042 unit/μg Chl a,respectively.However,most colonies resulting from the gene transfer showed no activity.PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies.Expression of the green fluorescent protein gene from the same promoter in Anabaena sp.PCC 7120 showed similar results.These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic engineering of Anabaena and Nostoc species.For the first time,we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium.The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites.
KeywordCyanobacteria
DepartmentState Key Laboratory for Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences;Graduate School of the Chinese Academy of Sciences;
Funding OrganizationSupported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001)
Indexed BySCI
Language英语
Funding OrganizationSupported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001) ; Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2002AA601170);the National Natural Science Foundation of China (No. 30623001)
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/14252
Collection藻类生物学及应用研究中心_期刊论文
Recommended Citation
GB/T 7714
Li Q,Tang Q,Xu XD,et al. Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria[J]. Chinese Journal of Oceanology and Limnology,2010(6).
APA 李琼,唐蜻,徐旭东,&高宏.(2010).Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria.Chinese Journal of Oceanology and Limnology(6).
MLA 李琼,et al."Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria".Chinese Journal of Oceanology and Limnology .6(2010).
Files in This Item:
File Name/Size DocType Version Access License
Expressionoforganoph(761KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[李琼]'s Articles
[唐蜻]'s Articles
[徐旭东]'s Articles
Baidu academic
Similar articles in Baidu academic
[李琼]'s Articles
[唐蜻]'s Articles
[徐旭东]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[李琼]'s Articles
[唐蜻]'s Articles
[徐旭东]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Expressionoforganopho_省略_n_fixingcyanoba.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.