IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 会议论文
Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio)
Xu, Zhuwei; Li, Qi; Wang, Yun; Song, Chaojun; Zhang, Tao; Chen, Lihua; Ji, Jianguo; Yang, Angang; Zhu, Zuoyan; Hu, Wei; Jin, Boquan; Hu, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Conference Name2nd International Symposium for Fish Growth and Reporduction
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
Pages373-378
Conference DateJUN 20-21, 2009
Conference PlaceHong Kong, PEOPLES R CHINA
Publication PlaceSAN DIEGO
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN0016-6480
Department[Wang, Yun; Zhu, Zuoyan; Hu, Wei] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Xu, Zhuwei; Li, Qi; Song, Chaojun; Chen, Lihua; Yang, Angang; Jin, Boquan] Fourth Mil Med Univ, Dept Immunol, Xian 710032, Peoples R China; [Zhang, Tao] Fourth Mil Med Univ, Tangdu Hosp, Dept Neurosurg, Xian 710038, Peoples R China; [Ji, Jianguo] Peking Univ, Sch Life Sci, Beijing, Peoples R China
AbstractThe gonadotropin alpha subunit (cGTH alpha), gonadotropinII beta subunit (cGTHII beta), somatolactin (cSL), and prolactin (cPRL) were isolated from the pituitaries of common carps, purified by traditional chromatographic analysis, identified by mass-chromatographic analysis, and used as immunogens in the B-lymphocyte hybridoma technique. Totally, 7, 11, 17, and 8 hybridoma cell lines were established, which were able to stably secrete monoclonal antibodies (mAbs) against cGTH alpha, cGTHII beta, cSL, and cPRL, and designated as FMU-cGTH alpha 1-7, FMU-cGTHII beta 1-11, FMU-cSL 1-17, and FMU-cPRL 1-8, respectively. The isotype, titer, and specificity were identified by enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemical staining, respectively, and application of these mAbs in the aforementioned tests has been proved. Furthermore, sensitive sandwich-ELISA systems for quantitative detection of the hormones mentioned above were also developed. (C) 2009 Elsevier Inc. All rights reserved.
KeywordCommon Carp Elisa Gonadotropin Monoclonal Antibody Prolactin Somatolactin
Indexed BySCI
Language英语
WOS IDWOS:000278875100007
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13874
Collection鱼类生物学及渔业生物技术研究中心_会议论文
Corresponding AuthorHu, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xu, Zhuwei,Li, Qi,Wang, Yun,et al. Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio)[C]. SAN DIEGO:ACADEMIC PRESS INC ELSEVIER SCIENCE,2010:373-378.
Files in This Item:
File Name/Size DocType Version Access License
Production, characte(460KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Zhuwei]'s Articles
[Li, Qi]'s Articles
[Wang, Yun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Zhuwei]'s Articles
[Li, Qi]'s Articles
[Wang, Yun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Zhuwei]'s Articles
[Li, Qi]'s Articles
[Wang, Yun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Production, characterization, and applications of mouse monoclonal antibodies.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.