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题名: Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio)
作者: Xu, Zhuwei ; Li, Qi ; Wang, Yun ; Song, Chaojun ; Zhang, Tao ; Chen, Lihua ; Ji, Jianguo ; Yang, Angang ; Zhu, Zuoyan ; Hu, Wei ; Jin, Boquan
会议文集: GENERAL AND COMPARATIVE ENDOCRINOLOGY
会议名称: 2nd International Symposium for Fish Growth and Reporduction
会议日期: JUN 20-21, 2009
出版日期: 2010
会议地点: Hong Kong, PEOPLES R CHINA
关键词: Common carp ; ELISA ; Gonadotropin ; Monoclonal antibody ; Prolactin ; Somatolactin
通讯作者: Hu, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
出版者: ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地: SAN DIEGO
收录类别: SCI
ISSN: 0016-6480
部门归属: [Wang, Yun; Zhu, Zuoyan; Hu, Wei] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Xu, Zhuwei; Li, Qi; Song, Chaojun; Chen, Lihua; Yang, Angang; Jin, Boquan] Fourth Mil Med Univ, Dept Immunol, Xian 710032, Peoples R China; [Zhang, Tao] Fourth Mil Med Univ, Tangdu Hosp, Dept Neurosurg, Xian 710038, Peoples R China; [Ji, Jianguo] Peking Univ, Sch Life Sci, Beijing, Peoples R China
摘要: The gonadotropin alpha subunit (cGTH alpha), gonadotropinII beta subunit (cGTHII beta), somatolactin (cSL), and prolactin (cPRL) were isolated from the pituitaries of common carps, purified by traditional chromatographic analysis, identified by mass-chromatographic analysis, and used as immunogens in the B-lymphocyte hybridoma technique. Totally, 7, 11, 17, and 8 hybridoma cell lines were established, which were able to stably secrete monoclonal antibodies (mAbs) against cGTH alpha, cGTHII beta, cSL, and cPRL, and designated as FMU-cGTH alpha 1-7, FMU-cGTHII beta 1-11, FMU-cSL 1-17, and FMU-cPRL 1-8, respectively. The isotype, titer, and specificity were identified by enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemical staining, respectively, and application of these mAbs in the aforementioned tests has been proved. Furthermore, sensitive sandwich-ELISA systems for quantitative detection of the hormones mentioned above were also developed. (C) 2009 Elsevier Inc. All rights reserved.
语种: 英语
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内容类型: 会议论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13874
Appears in Collections:鱼类生物学及渔业生物技术研究中心_会议论文

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Xu, Zhuwei; Li, Qi; Wang, Yun; Song, Chaojun; Zhang, Tao; Chen, Lihua; Ji, Jianguo; Yang, Angang; Zhu, Zuoyan; Hu, Wei; Jin, Boquan.Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio).见:ACADEMIC PRESS INC ELSEVIER SCIENCE.GENERAL AND COMPARATIVE ENDOCRINOLOGY,SAN DIEGO,2010,373-378
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文件名: Production, characterization, and applications of mouse monoclonal antibodies.pdf
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