中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity
题名: Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53
作者: Su, F.1; Pascal, L. E.1; Xiao, W.2; Wang, Z.1, 3
通讯作者: Wang, Z, Univ Pittsburgh, Sch Med, Inst Canc, Dept Urol, 5200 Ctr Ave,Suite G40, Pittsburgh, PA 15232 USA
关键词: U19/EAF2 ; prostate cancer ; tumor suppressor ; TSP-1 ; p53
刊名: ONCOGENE
发表日期: 2010-01-21
DOI: 10.1038/onc.2009.326
卷: 29, 期:3, 页:421-431
收录类别: SCI
文章类型: Article
部门归属: [Su, F.; Pascal, L. E.; Wang, Z.] Univ Pittsburgh, Sch Med, Inst Canc, Dept Urol, Pittsburgh, PA 15232 USA; [Xiao, W.] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China; [Wang, Z.] Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15232 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NIH [R01 CA 120386, R37 DK51993]; Prostate Cancer Specialized Program of Research Excellence (SPORE) [P50 CA90386]
类目[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
摘要: Inactivation of U19/EAF2 has been shown previously to lead to tumorigenesis in multiple organs; however, the mechanism of U19/EAF2 tumor suppression remains unclear. In this paper, we report that the expression of an anti-angiogenic protein, thrombospondin-1 (TSP-1) is down-regulated in the prostate and liver of U19/EAF2 knockout mouse. The U19/EAF2 knockout liver displayed increased CD31-positive blood vessels, suggesting that the TSP-1 down-regulation can contribute to increased angiogenesis. TSP-1 is reported to be a p53-target gene and p53 is a known binding partner of ELL, which binds to U19/EAF2. Here, we show that U19/EAF2 can co-localize and co-immunoprecipitate with p53 in transfected cells. In a TSP-1 promoter-driven luciferase reporter assay, p53 transfection suppressed the TSP-1 promoter activity and U19/EAF2 co-transfection blocked the p53 suppression of TSP-1 promoter. However, U19/EAF2 transfection alone had little or no effect on the TSP-1 promoter. The above observations together suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. Oncogene (2010) 29, 421-431; doi:10.1038/onc.2009.326; published online 12 October 2009
英文摘要: Inactivation of U19/EAF2 has been shown previously to lead to tumorigenesis in multiple organs; however, the mechanism of U19/EAF2 tumor suppression remains unclear. In this paper, we report that the expression of an anti-angiogenic protein, thrombospondin-1 (TSP-1) is down-regulated in the prostate and liver of U19/EAF2 knockout mouse. The U19/EAF2 knockout liver displayed increased CD31-positive blood vessels, suggesting that the TSP-1 down-regulation can contribute to increased angiogenesis. TSP-1 is reported to be a p53-target gene and p53 is a known binding partner of ELL, which binds to U19/EAF2. Here, we show that U19/EAF2 can co-localize and co-immunoprecipitate with p53 in transfected cells. In a TSP-1 promoter-driven luciferase reporter assay, p53 transfection suppressed the TSP-1 promoter activity and U19/EAF2 co-transfection blocked the p53 suppression of TSP-1 promoter. However, U19/EAF2 transfection alone had little or no effect on the TSP-1 promoter. The above observations together suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. Oncogene (2010) 29, 421-431; doi:10.1038/onc.2009.326; published online 12 October 2009
关键词[WOS]: HIPPEL-LINDAU PROTEIN ; ELONGATION-FACTOR ELL ; PROSTATE-CANCER ; GENE ; ANGIOGENESIS ; GROWTH ; CELLS ; CARCINOMA ; APOPTOSIS ; TRANSACTIVATION
语种: 英语
WOS记录号: WOS:000273793200011
ISSN号: 0950-9232
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13860
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
20480_ftp.pdf(247KB)----开放获取--View 联系获取全文

作者单位: 1.Univ Pittsburgh, Sch Med, Inst Canc, Dept Urol, Pittsburgh, PA 15232 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
3.Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15232 USA

Recommended Citation:
Su, F.; Pascal, L. E.; Xiao, W.; Wang, Z..Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53,ONCOGENE,2010,29(3):421-431
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Su, F.]'s Articles
[Pascal, L. E.]'s Articles
[Xiao, W.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Su, F.]‘s Articles
[Pascal, L. E.]‘s Articles
[Xiao, W.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 20480_ftp.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace